Anti-Thrombospondin 1抗体(ab88529)
Key features and details
- Mouse polyclonal to Thrombospondin 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Thrombospondin 1抗体
参阅全部 Thrombospondin 1 一抗 -
描述
小鼠多克隆抗体to Thrombospondin 1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human Thrombospondin 1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
阳性对照
- WB: Human pancreas tissue lysate and Thrombospondin transfected 293T cell lysate IF: HeLa cell
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88529于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa.
|
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 129 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
-
功能
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors. -
序列相似性
Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. -
细胞定位
Endoplasmic reticulum. Sarcoplasmic reticulum. - Information by UniProt
-
数据库链接
- Entrez Gene: 7057 Human
- Omim: 188060 Human
- SwissProt: P07996 Human
- Unigene: 164226 Human
- Unigene: 732539 Human
-
别名
- Thbs1 antibody
- Thrombospondin-1 antibody
- TSP antibody
see all
图片
-
All lanes : Anti-Thrombospondin 1 antibody (ab88529) at 1 µg/ml
Lane 1 : Thrombospondin transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 129 kDa -
ab88529 at 10 µg/ml staining Thrombospondin in paraformaldehyde fixed, permeabilised HeLa cells, by immunofluorescence.
-
Anti-Thrombospondin 1 antibody (ab88529) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
Predicted band size: 129 kDa
Observed band size: ~ 150 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
-
Datasheet download
文献 (6)
ab88529 被引用在 6 文献中.
- Guo L et al. Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion. Theranostics 10:6581-6598 (2020). PubMed: 32550891
- Liu Y et al. MicroRNA-182-5p contributes to the protective effects of thrombospondin 1 against lipotoxicity in INS-1 cells. Exp Ther Med 16:5272-5279 (2018). PubMed: 30546417
- Duan S et al. Effects of taxol resistance gene 1 on the cisplatin response in gastric cancer. Oncol Lett 15:8287-8294 (2018). PubMed: 29805561
- Patel GK et al. Exosomes confer chemoresistance to pancreatic cancer cells by promoting ROS detoxification and miR-155-mediated suppression of key gemcitabine-metabolising enzyme, DCK. Br J Cancer 116:609-619 (2017). PubMed: 28152544
- Xu C et al. Circadian Clock Regulates Bone Resorption in Mice. J Bone Miner Res 31:1344-55 (2016). PubMed: 26841172
- Bonazzi VF et al. Cross-Platform Array Screening Identifies COL1A2, THBS1, TNFRSF10D and UCHL1 as Genes Frequently Silenced by Methylation in Melanoma. PLoS One 6:e26121 (2011). WB ; Human . PubMed: 22028813