概述

  • 产品名称
    Anti-Thrombospondin 2抗体
    参阅全部 Thrombospondin 2 一抗
  • 描述
    兔多克隆抗体to Thrombospondin 2
  • 宿主
    Rabbit
  • 特异性
    Reactivity in Rat is no longer guaranteed due to difficulty reproducing in-house experimental evidence, but will remain predicted, due to the immunogen showing 100% xr with Rat.
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human Thrombospondin 2.

    (Peptide available as ab96113.)

  • 阳性对照
    • This antibody gave a positive signal in both Human and Mouse spinal cord tissue lysate. This antibody gave a positive signal in ICC/IF in U2O2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab84469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

图片

  • Ab84469 staining Thrombospondin 2 in U2O2 cells. The cells were fixed with 100% Methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab84469 at 10ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and tubulin was labelled using ab7291 Mouse monoclonal to alpha Tubulin together with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594), at a 1/1000 dilution (shown in red).

  • All lanes : Anti-Thrombospondin 2 antibody (ab84469) at 1 mg/ml

    Lane 1 : Mouse spinal cord embryo E14
    Lane 2 : Human skin
    Lane 3 : Mouse skin
    Lane 4 : A431 (Human epithelial carcinoma cell line)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 129 kDa
    Additional bands at: 210 kDa (possible glycosylated form), 46 kDa (possible cleavage fragment), 58 kDa (possible non-specific binding)


    Exposure time: 20 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    ab84469 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

文献

This product has been referenced in:
  • Wang S  et al. Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure. PLoS One 12:e0185388 (2017). Read more (PubMed: 28953973) »

See 1 Publication for this product

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