Anti-Thrombospondin 2抗体(ab84469)
Key features and details
- Rabbit polyclonal to Thrombospondin 2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Thrombospondin 2抗体
参阅全部 Thrombospondin 2 一抗 -
描述
兔多克隆抗体to Thrombospondin 2 -
宿主
Rabbit -
特异性
Reactivity in Rat is no longer guaranteed due to difficulty reproducing in-house experimental evidence, but will remain predicted, due to the immunogen showing 100% xr with Rat. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Horse, Chicken, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human Thrombospondin 2 aa 650-750 conjugated to keyhole limpet haemocyanin.
(Peptide available asab96113) -
阳性对照
- This antibody gave a positive signal in both Human and Mouse spinal cord tissue lysate. This antibody gave a positive signal in ICC/IF in U2O2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84469于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 129 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. -
组织特异性
High expression in invertebral disk tissue. -
疾病相关
Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. -
序列相似性
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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数据库链接
- Entrez Gene: 414837 Chicken
- Entrez Gene: 338092 Cow
- Entrez Gene: 7058 Human
- Entrez Gene: 21826 Mouse
- Entrez Gene: 292406 Rat
- Omim: 188061 Human
- SwissProt: P35440 Chicken
- SwissProt: Q95116 Cow
see all -
别名
- THBS2 antibody
- Thrombospondin-2 antibody
- TSP2 antibody
- TSP2_HUMAN antibody
图片
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Ab84469 staining Thrombospondin 2 in U2O2 cells. The cells were fixed with 100% Methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab84469 at 10ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and tubulin was labelled using ab7291 Mouse monoclonal to alpha Tubulin together with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594), at a 1/1000 dilution (shown in red).
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All lanes : Anti-Thrombospondin 2 antibody (ab84469) at 1 mg/ml
Lane 1 : Mouse spinal cord embryo E14
Lane 2 : Human skin
Lane 3 : Mouse skin
Lane 4 : A431 (Human epithelial carcinoma cell line)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 129 kDa
Additional bands at: 210 kDa (possible glycosylated form), 46 kDa (possible cleavage fragment), 58 kDa (possible non-specific binding)
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with abX overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab84469 contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab84469 被引用在 11 文献中.
- Li Y et al. Circ-OPHN1 suppresses the proliferation, migration, and invasion of trophoblast cells through mediating miR-558/THBS2 axis. Drug Dev Res 83:1034-1046 (2022). PubMed: 35277867
- Liu J et al. circ_0085296 inhibits the biological functions of trophoblast cells to promote the progression of preeclampsia via the miR-942-5p/THBS2 network. Open Med (Wars) 17:577-588 (2022). PubMed: 35415249
- Hu T et al. Astrocyte derived TSP2 contributes to synaptic alteration and visual dysfunction in retinal ischemia/reperfusion injury. Cell Biosci 12:196 (2022). PubMed: 36471420
- Ye Y et al. Circ-AK2 is associated with preeclampsia and regulates biological behaviors of trophoblast cells through miR-454-3p/THBS2. Placenta 103:156-163 (2021). PubMed: 33129036
- Rashid MU et al. Zika virus dysregulates human Sertoli cell proteins involved in spermatogenesis with little effect on tight junctions. PLoS Negl Trop Dis 14:e0008335 (2020). PubMed: 32511241