重组Anti-Thrombopoietin抗体[EPR14948] (ab196026)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14948] to Thrombopoietin
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Thrombopoietin抗体[EPR14948]
参阅全部 Thrombopoietin 一抗 -
描述
兔单克隆抗体[EPR14948] to Thrombopoietin -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal liver; Thrombopoietin recombinant protein; Human liver and bone marrow tissues; Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14948 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab196026于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/40.
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WB |
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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ICC/IF |
1/50.
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/40. |
WB
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
1/50. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. -
疾病相关
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. -
序列相似性
Belongs to the EPO/TPO family. -
结构域
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7066 Human
- Omim: 600044 Human
- SwissProt: P40225 Human
- Unigene: 1166 Human
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别名
- C mpl ligand antibody
- C-mpl ligand antibody
- Megakaryocyte colony stimulating factor antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cytoplasm staining on human bone marrow tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Thrombopoietin with purified ab196026 at 1/40 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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Immunofluorescent analysis of Jurkat cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Thrombopoietin with ab196026 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
Note: Cytoplasm staining on Jurkat cell line was observed.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cytoplasm staining on human liver tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/5000 dilution + Thrombopoietin recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa -
Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/1000 dilution + Human fetal liver at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 38 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (7)
ab196026 被引用在 7 文献中.
- Yang L et al. Generation of a thrombopoietin-deficient thrombocytopenia model in zebrafish. J Thromb Haemost 20:1900-1909 (2022). PubMed: 35622056
- Wang X et al. Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells. Cancer Gene Ther 29:1742-1750 (2022). PubMed: 35768562
- Rani GF et al. Hematological consequences of malaria in mice previously treated for visceral leishmaniasis. Wellcome Open Res 6:83 (2021). PubMed: 34286101
- Zou Z et al. Endogenous thrombopoietin promotes non-small-cell lung carcinoma cell proliferation and migration by regulating EGFR signalling. J Cell Mol Med 24:6644-6657 (2020). PubMed: 32337844
- Zhou CL et al. Thrombopoietin is associated with a prognosis of gastric adenocarcinoma. Rev Assoc Med Bras (1992) 66:590-595 (2020). PubMed: 32638965
- Yan Z et al. CD110 promotes pancreatic cancer progression and its expression is correlated with poor prognosis. J Cancer Res Clin Oncol 145:1147-1164 (2019). PubMed: 30770989
- Pecci A et al. Thrombopoietin mutation in congenital amegakaryocytic thrombocytopenia treatable with romiplostim. EMBO Mol Med 10:63-75 (2018). PubMed: 29191945