概述

  • 产品名称Anti-Thrombopoietin抗体
    参阅全部 Thrombopoietin 一抗
  • 描述
    兔多克隆抗体to Thrombopoietin
  • 特异性Cross reactivity: Thrombopoietin Human (299-332) 100%; Thrombopoietin Human recombinant ~90% Does not cross react with Erythropoietin Human recombinant.
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide: PPTGQYTLFP LPPTLPTPVV QLHPLLPDPS APTP conjugated to KLH, corresponding to amino acids 299-332 of Human Thrombopoietin Peptide available as ab93626.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab40664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500.
IHC-P Use at an assay dependent concentration. PubMed: 21149592
WB 1/800. Detects a band of approximately 59 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human Thrombopoietin peptide (ab93626).
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

靶标

  • 功能Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
  • 疾病相关Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
  • 序列相似性Belongs to the EPO/TPO family.
  • 结构域Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C mpl ligand antibody
    • C-mpl ligand antibody
    • Megakaryocyte colony stimulating factor antibody
    • Megakaryocyte colony-stimulating factor antibody
    • Megakaryocyte growth and development factor antibody
    • Megakaryocyte stimulating factor antibody
    • MGC163194 antibody
    • MGDF antibody
    • MKCSF antibody
    • ML antibody
    • MPL ligand antibody
    • MPLLG antibody
    • Myeloproliferative leukemia virus oncogene ligand antibody
    • Prepro thrombopoietin antibody
    • THCYT1 antibody
    • THPO antibody
    • Thrombopoietin antibody
    • Thrombopoietin nirs variant 1 antibody
    • TPO antibody
    • TPO_HUMAN antibody
    see all

Anti-Thrombopoietin antibody (ab40664)参考文献

This product has been referenced in:
  • Hoffmann O  et al. Thrombopoietin contributes to neuronal damage in experimental bacterial meningitis. Infect Immun 79:928-36 (2011). IHC-P ; Human . Read more (PubMed: 21149592) »

See 1 Publication for this product

Product Wall

Thank you for your reply. If I am understanding your assay correctly, your cells are overexpressing a thrombopoietin receptor and you are incubating the cell culture with recombinant thrombopoietin to measure the binding efficacy. What type of recombin...

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Thank you for your reply and the additional information.

Regarding the refund, you would need to let your purchasing department know the information I had sent and they then should contact us so that the refund can be issued to you. I'm certai...

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Thank you for contacting us.
I am sorry to hear that these antibodies do not work as expected.

According to the records in our system, the purchase of ab40664 has already been refunded and the case has been discussed and resolved.

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Your credit note ID for the refund is xxx.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note ...

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It's OK if you do no have any images at hand.
You protocol looks fine, thus it seems the antibody is not working as it should - either the vial or the lot.

Please let me know your order or PO number. I'd be...

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Thank you for contacting us.

I'm sorry to hear the antibody is not working as expected.
I have not heard of this problem before for this antibody, in fact this is the first complaint we receive for this product.

Could you plea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"