Anti-Thrombomodulin抗体[PBS-02] (ab7640)


  • 产品名称Anti-Thrombomodulin抗体[PBS-02]
    参阅全部 Thrombomodulin 一抗
  • 描述
    小鼠单克隆抗体[PBS-02] to Thrombomodulin
  • 经测试应用适用于: WB, ELISA, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

  • 表位EGF domain 2 of thrombomodulin.



Our Abpromise guarantee covers the use of ab7640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells.


  • 功能Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • 组织特异性Endothelial cells are unique in synthesizing thrombomodulin.
  • 疾病相关Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • 序列相似性Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • 翻译后修饰N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AHUS 6 antibody
    • AHUS6 antibody
    • BDCA 3 antibody
    • BDCA3 antibody
    • CD 141 antibody
    • CD141 antibody
    • CD141 antigen antibody
    • Fetomodulin antibody
    • Thbd antibody
    • THPH12 antibody
    • THRM antibody
    • Thrombomodulin antibody
    • TM antibody
    • TRBM_HUMAN antibody
    see all

Anti-Thrombomodulin antibody [PBS-02] 图像

  • ab7640 (1µg/ml) staining thrmbomodulin in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of pneumocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing THP1 cells stained with ab7640 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7640, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Thrombomodulin antibody [PBS-02] (ab7640)参考文献

This product has been referenced in:
  • Lee EJ & Niklason LE A novel flow bioreactor for in vitro microvascularization. Tissue Eng Part C Methods 16:1191-200 (2010). WB ; Rat . Read more (PubMed: 20170423) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human umbilical vein endothelial cells)
Loading amount 30 µg
Specification Human umbilical vein endothelial cells
Gel Running Conditions Reduced Denaturing (10%`)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Apr 17 2008

Thank you for your enquiry. We don't sell the truncated recombinant thrombomodulin used as the standard in the ELISA demonstration on the online datasheet. Please contact us again if you require further information to that provided on the datasheet.

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in re...

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Try this antibody at 1:1000 and adjust the dilution accordingly from the results obtained.

Yes, ab7640 antibody recognises EGF domain 2 which is located within the extracellular domain of this integral membrane protein (EGF domains are regions containing sequence homologous to human epidermal growth factor). The antibody has been shown to wo...

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