The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
功能High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
组织特异性Platelets and vascular endothelial cells.
序列相似性Belongs to the G-protein coupled receptor 1 family.
翻译后修饰A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor.