Anti-Thrombin抗体[BDI905] (ab20877)


  • 产品名称Anti-Thrombin抗体[BDI905]
    参阅全部 Thrombin 一抗
  • 描述
    小鼠单克隆抗体[BDI905] to Thrombin
  • 特异性Ab20877 has not been tested for cross-reactivity with prothrombin therefore we do not know if it will cross react with it.
  • 经测试应用适用于: ICC/IF, ELISAmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (purified) (Human)



Our Abpromise guarantee covers the use of ab20877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent dilution.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. The molecular mass of thrombin is 37 kDa.
    • 细胞定位Secreted
    • 数据库链接
    • 别名
      • Coagulation factor II antibody
      • Coagulation factor II thrombin antibody
      • EC antibody
      • F2 antibody
      • Factor II antibody
      • Prothrombin B chain antibody
      • PT antibody
      • Serine protease antibody
      see all

    Anti-Thrombin antibody [BDI905] 图像

    • ICC/IF image of ab20877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20877, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-goat mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

    Anti-Thrombin antibody [BDI905] (ab20877)参考文献

    ab20877 has not yet been referenced specifically in any publications.

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