Anti-Thrombin抗体[BDI905] (ab20877)

概述

  • 产品名称Anti-Thrombin抗体[BDI905]
    参阅全部 Thrombin 一抗
  • 描述
    小鼠单克隆抗体[BDI905] to Thrombin
  • 特异性Ab20877 has not been tested for cross-reactivity with prothrombin therefore we do not know if it will cross react with it.
  • 经测试应用适用于: ICC/IF, ELISAmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (purified) (Human)

性能

应用

Our Abpromise guarantee covers the use of ab20877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent dilution.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
    • 组织特异性Expressed by the liver and secreted in plasma.
    • 疾病相关Factor II deficiency
      Ischemic stroke
      Thrombophilia due to thrombin defect
      Pregnancy loss, recurrent, 2
    • 序列相似性Belongs to the peptidase S1 family.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.
    • 翻译后修饰The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
      N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
    • 细胞定位Secreted, extracellular space.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Coagulation factor II antibody
      • Coagulation factor II thrombin antibody
      • EC 3.4.21.5 antibody
      • F2 antibody
      • Factor II antibody
      • Prepro coagulation factor II antibody
      • Prothrombin antibody
      • Prothrombin B chain antibody
      • PT antibody
      • RPRGL2 antibody
      • Serine protease antibody
      • THPH1 antibody
      • THRB_HUMAN antibody
      • Thrombin heavy chain antibody
      see all

    Anti-Thrombin antibody [BDI905] 图像

    • ICC/IF image of ab20877 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20877, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-goat mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

    Anti-Thrombin antibody [BDI905] (ab20877)参考文献

    ab20877 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"