Anti-Thrombin抗体(ab83981)

概述

  • 产品名称Anti-Thrombin抗体
    参阅全部 Thrombin 一抗
  • 描述
    兔多克隆抗体to Thrombin
  • 经测试应用适用于: ICC/IF, IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 396 - 445 (ISDRWVLTAA HCLLYPPWDK NFTENDLLVR IGKHSRTRYE RNIEKISMLE) of human Thrombin (NP_000497).

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Factor II deficiency
    Ischemic stroke
    Thrombophilia due to thrombin defect
    Pregnancy loss, recurrent, 2
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
  • 细胞定位Secreted, extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coagulation factor II antibody
    • Coagulation factor II thrombin antibody
    • EC 3.4.21.5 antibody
    • F2 antibody
    • Factor II antibody
    • Prepro coagulation factor II antibody
    • Prothrombin antibody
    • Prothrombin B chain antibody
    • PT antibody
    • RPRGL2 antibody
    • Serine protease antibody
    • THPH1 antibody
    • THRB_HUMAN antibody
    • Thrombin heavy chain antibody
    see all

Anti-Thrombin antibody 图像

  • ab83981 staining Thrombin in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/500 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-Thrombin antibody (ab83981) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 70 kDa
    Observed band size : 70 kDa
  • ab83981 at 5 µg/ml staining Thrombin in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab83981 at 5 µg/ml staining Thrombin in Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ICC/IF image of ab83981 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83981, 5µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Thrombin antibody (ab83981)参考文献

ab83981 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (Human lung)
Specification Human lung
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 07 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human liver)
Specification human liver
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 11 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Oct 10 2012

DISCOUNT CODE: xxxxxxx
Expiration date: 15th of December 2012

I am very pleased to hear you would like to accept our offer and test ab83981 in IHC with frozen sections. Thecode above will give you: 1 free primary antibody or the value of...

Read More

Thank you for contacting us and your interest in our products.

Unfortunately, as can be seen from the datasheet of the anti-Thrombin antibody (ab83981), we have currently only tried this antibody with IHC of paraffin embedded tissue sections....

Read More

Thank you for contacting us.

The link to the protein database entry for the human thrombin protein is as follows:
http://www.uniprot.org/uniprot/P00734

I hope this information is helpful to you. Please do not hesitate to cont...

Read More

Thank you for contacting us. Based on the peptide immunogen sequence, this antibody will cross react with pro-thrombin (http://www.uniprot.org/uniprot/P00734).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"