• 产品名称Anti-Thrombin抗体
    参阅全部 Thrombin 一抗
  • 描述
    兔多克隆抗体to Thrombin
  • 特异性The antibody will recognize prothrombin and thrombin
  • 经测试应用适用于: IHC-P, RIA, IP, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein: Human thrombin purified from human plasma

  • 阳性对照
    • IHC-P: Human liver FFPE tissue sections.



Our Abpromise guarantee covers the use of ab48626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
RIA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.


  • 相关性Thrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. The molecular mass of thrombin is 37 kDa.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • Coagulation factor II antibody
    • Coagulation factor II thrombin antibody
    • EC antibody
    • F2 antibody
    • Factor II antibody
    • Prothrombin B chain antibody
    • PT antibody
    • Serine protease antibody
    see all

Anti-Thrombin antibody 图像

  • IHC image of Thrombin staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond  system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48626, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab48626 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48626, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Thrombin antibody (ab48626)参考文献

ab48626 has not yet been referenced specifically in any publications.

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

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Thank you for contacting me again. Detecting thrombin specifically is a very hard issue. As thrombin is part of pro thrombin and the proform undergoes activation during blood coagulation therefore it is difficult to differentiate both proteins....

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