THP1核extract裂解液(PMA stimulated) (ab14901)

概述

  • 产品名称THP1核extract裂解液(PMA stimulated)
    参阅全部 THP1 细胞裂解液
  • 描述
    THP1核extract裂解液
  • 常规说明Cell line: THP1 (Human acute monocytic leukemia).
    To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
    The protein content has been determined by a Bradford-based assay.
    The THP-1 nuclear extracts have been collected in Lysis Buffer after a 24-hour incubation with or without PMA (10 ng/ml).

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
    Note: With Protease inhibitors.
  • Concentration information loading...
  • 研究领域
  • 背景Properties: differentiation; lysozyme synthesis; phagocytosis; Fc receptor; IL-1 prod.; C3b receptor. The cell line can differentiate into macrophage-like cells (phagocytic) and can be used for induction of differentiation studies. When stimulated with PMA cells have been reported to differentialte into a monocytic pathway and release arachidonic acid and prostanoids.

THP1 nuclear extract lysate (PMA stimulated) 图像

  • Each lot has been tested for PPAR-gamma activation by using TransAM™ PPAR-gamma Kits. The signal intensity for PPAR-gamma activation in each lot is compared to the signal intensity obtained with extracts from unstimulated THP-1 cells (see figure). After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of PPAR-gamma activation in a given cell type.

THP1 nuclear extract lysate (PMA stimulated) (ab14901)参考文献

ab14901 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. For the stimulation of THP1 cells with PMA please perform the following; Grow THP-1 cells at a density range of 5-8 x 10exp5 c/ml., add PMA to a final concentration of 10 ng/ml to stimulate the cells. Do not add anyt...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"