Anti-Thomsen-Friedenreich Antigen抗体[A78-G/A7] (ab80553)

概述

  • 产品名称Anti-Thomsen-Friedenreich Antigen抗体[A78-G/A7]
    参阅全部 Thomsen-Friedenreich Antigen 一抗
  • 描述
    小鼠单克隆抗体[A78-G/A7] to Thomsen-Friedenreich Antigen
  • 特异性ab80553 is specific for both anomeric forms of the disaccharide (TFalpha and TFbeta, including related structures on the glycolipid) and shows no cross reactivity with sialylated glycophorin.
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Neuraminidase-treated human red blood cells.

  • 表位Galß1-3GalNAc
  • 阳性对照
    • KG1 cells. Human colorectal carcinoma tissue. Human colon adenocarcinoma tissue.
  • 常规说明


    ab80553 can be applied for the detection of cells with Thomsen-Friedenreich antigen and is especially applicable for sensitive determination of neuraminidases. It can be used on frozen and formalin fixed, paraffin fixed tissues and is capable of agglutinating desialylated red blood cells (Karsten et al, 1995).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明Purified from the ascites fluid by ammonium sulfate precipitation.
  • Primary antibody说明ab80553 can be applied for the detection of cells with Thomsen-Friedenreich antigen and is especially applicable for sensitive determination of neuraminidases. It can be used on frozen and formalin fixed, paraffin fixed tissues and is capable of agglutinating desialylated red blood cells (Karsten et al, 1995).
  • 克隆单克隆
  • 克隆编号A78-G/A7
  • 同种型IgM
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

1/25 - 1/50 for 30 minutes at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性The Thomsen Friedenreich antigen acts as an oncofoetal antigen, with low expression in normal adult tissues but increasing to foetal levels of expression in hyperplasia or malignancy. It is considered as a pan carcinoma marker. Can be applied for the detection of cells with TF antigen and is especially applicable for sensitive determination of neuraminidases.
  • 细胞定位Cell Membrane
  • 别名
    • CD176 antibody
    • TF antibody
    • TF antigen antibody

Anti-Thomsen-Friedenreich Antigen antibody [A78-G/A7] (ab80553)参考文献

This product has been referenced in:
  • Baldus SE  et al. Coexpression of MUC1 mucin peptide core and the Thomsen-Friedenreich antigen in colorectal neoplasms. Cancer 82:1019-27 (1998). Read more (PubMed: 9506345) »
  • Karsten U  et al. A new monoclonal antibody (A78-G/A7) to the Thomsen-Friedenreich pan-tumor antigen. Hybridoma 14:37-44 (1995). Read more (PubMed: 7539400) »

See all 2 Publications for this product

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