Anti-THOC2抗体(ab46685)

概述

  • 产品名称Anti-THOC2抗体
    参阅全部 THOC2 一抗
  • 描述
    兔多克隆抗体to THOC2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human THOC2.

    (Peptides available as ab46868 and ab46869.)

  • 阳性对照
    • This antibody gave a positive signal in Hela Nuclear cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab46685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa).

靶标

  • 功能Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex.The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
  • 序列相似性Belongs to the THOC2 family.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CXorf3 antibody
    • DJ506G2.1 antibody
    • hTREX120 antibody
    • RP5 1189B24.5 antibody
    • THO complex 2 antibody
    • THO complex subunit 2 antibody
    • Tho2 antibody
    • THOC2 antibody
    • THOC2_HUMAN antibody
    see all

Anti-THOC2 antibody 图像

  • Anti-THOC2 antibody (ab46685) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 183 kDa
    Observed band size : 183 kDa
    Additional bands at : 130 kDa,35 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab46685 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab46685, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, Hek293 and MCF7 cells fixed in 4% PFA and HepG2 cells fixed in 100% methanol at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

Anti-THOC2 antibody (ab46685)参考文献

ab46685 has not yet been referenced specifically in any publications.

Product Wall

According to our records, ab46685 was proving difficult to use in WB and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the diffic...

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Thank you very much for contacting us. We have used Hela cells to test this antibody. In our hands this antibody showed a band at 183 kDa. However we have also seen some non specific bands at 130, 35 and 55 kda unfortunately we do not know the ident...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 May 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"