Anti-Thioredoxin / TRX抗体[3A1] (ab16965)

概述

  • 产品名称Anti-Thioredoxin / TRX抗体[3A1]
    参阅全部 Thioredoxin / TRX 一抗
  • 描述
    小鼠单克隆抗体[3A1] to Thioredoxin / TRX
  • 经测试应用适用于: Flow Cyt, ELISA, WB, IPmore details
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Human) purified from E.coli

  • 阳性对照
    • HeLa cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab16965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 12 kDa.
IP Use at an assay dependent concentration.

靶标

  • 功能Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
    ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
  • 序列相似性Belongs to the thioredoxin family.
    Contains 1 thioredoxin domain.
  • 翻译后修饰In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.
    In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
  • 细胞定位Nucleus. Cytoplasm. Secreted. Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADF antibody
    • ATL derived factor antibody
    • ATL-derived factor antibody
    • DKFZp686B1993 antibody
    • MGC61975 antibody
    • SASP antibody
    • Surface associated sulphydryl protein antibody
    • Surface-associated sulphydryl protein antibody
    • testicular tissue protein Li 199 antibody
    • THIO_HUMAN antibody
    • Thioredoxin antibody
    • thioredoxin delta 3 antibody
    • TRDX antibody
    • TRX 1 antibody
    • Trx antibody
    • TRX1 antibody
    • TXN antibody
    • TXN delta 3 antibody
    • TXN protein antibody
    • zgc:92903 antibody
    see all

Anti-Thioredoxin / TRX antibody [3A1] 图像

  • All lanes : Anti-Thioredoxin / TRX antibody [3A1] (ab16965) at 1/2000 dilution

    Lane 1 : Cell lysates prepared from Hela cells
    Lane 2 : Cell lysates prepared from Hela cells

    Lysates/proteins at 20 µg per lane.


  • Overlay histogram showing HeLa cells stained with ab16965 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16965, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Thioredoxin / TRX antibody [3A1] (ab16965)参考文献

This product has been referenced in:
  • Peeters PM  et al. Silica-induced NLRP3 inflammasome activation in vitro and in rat lungs. Part Fibre Toxicol 11:58 (2014). WB ; Human . Read more (PubMed: 25406505) »
  • Carilho Torrao RB  et al. Healthy ageing and depletion of intracellular glutathione influences T cell membrane thioredoxin-1 levels and cytokine secretion. Chem Cent J 7:150 (2013). Flow Cyt ; Human . Read more (PubMed: 24007191) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Amniotic Epithelial Cells)
Permeabilization Yes - Triton-X for 10 min
Specification Human Amniotic Epithelial Cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative Paraformaldehyde
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提交于 Oct 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"