Anti-Thioredoxin 2抗体- C-terminal (ab71262)

概述

  • 产品名称Anti-Thioredoxin 2抗体- C-terminal
    参阅全部 Thioredoxin 2 一抗
  • 描述
    兔多克隆抗体to Thioredoxin 2 - C-terminal
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide selected from the C-terminal region of human Thioredoxin 2 conjugated to KLH

  • 阳性对照
    • 293 cell line lysate. IHC-P: FFPE human testis tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab71262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/36 - 1/500. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
  • 细胞定位Mitochondrial
  • 数据库链接
  • 别名
    • mitochondrial thioredoxin antibody
    • MT-TRX antibody
    • MTRX antibody
    • thioredoxin 2 precursor antibody
    • Thioredoxin mitochondrial antibody
    • Thioredoxin2 antibody
    • TRX 2 antibody
    • TRX2 antibody
    • TXN 2 antibody
    • TXN2 antibody
    see all

Anti-Thioredoxin 2 antibody - C-terminal 图像

  • IHC image of Thioredoxin 2 staining in human testis formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71262, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-Thioredoxin 2 antibody - C-terminal (ab71262) at 1/36 dilution + 293 cell line lysate at 35 µg

    Predicted band size : 18 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

Anti-Thioredoxin 2 antibody - C-terminal (ab71262)参考文献

ab71262 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (cochlea, heart. liver, brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: .01 M sodimu citrate pH 6
Permeabilization Yes - tween 20
Specification cochlea, heart. liver, brain
Blocking step BSA, goat serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative Paraformaldehyde
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Prof. Paul Linser

Verified customer

提交于 Jun 23 2015

Thank you for your call today. I've substituted ab71262 for ab16856 on the order, and this should ship out tonight for delivery tomorrow.

DISCOUNT CODE: ***
Expiration date: August 11, 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"