The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The protein encoded by the THBS3 gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins which mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond and shares a common promoter with metaxin 1.
ab92340 (5µg/ml) shows staining of THBS3 in paraffin embedded Human Colon following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows endothelial cell staining of a blood vessel.