Synthetic peptide conjugated to KLH, corresponding to a region between N terminal amino acids 10-40 of Human THAP3.
MDA-MB435 cell line lysates and Human brain tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 27 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
THAP3 contains 1 THAP-type zinc finger. There are three known isoforms produced by alternative splicing.
Western blot - THAP3 antibody (ab103887)
Anti-THAP3 antibody (ab103887) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg
Predicted band size : 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - THAP3 antibody (ab103887)
ab103887, at 1/50 dilution, staining THAP3 in formalin-fixed, paraffin-embedded Human brain tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"