antigen sequence: KSNISSQQVL LEHSYAFRNP MEAKKRIIKL EKEIASLRRK MKTCLQKERR ATRRWIKATC LVKNLEANSV LPKGTSEHML PTALSSLPLE D, corresponding to amino acids 112-202 of Human THAP2 (Q9H0W7).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Contains 1 THAP-type zinc finger.
Information by UniProt
THAP domain-containing protein 2 antibody
Immunocytochemistry/ Immunofluorescence - Anti- THAP2 antibody (ab122588)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and golgi apparatus. Recommended concentration of ab122588 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab122588)
ab122588, at 1/20 dilution, staining human stomach by immunohistochemistry of paraffin embedded tissues (IHC-P).
has not yet been referenced specifically in any publications.
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