The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Transcriptional repressor that plays a central role for embryogenesis and the pluripotency of embryonic stem (ES) cells. Sequence-specific DNA-binding factor that represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers.
序列相似性Belongs to the THAP11 family. Contains 1 THAP-type zinc finger.
细胞定位Nucleus. Cytoplasm. May be regulated by shuttling of the protein between the cytoplasm and nucleus.
Detection of THAP11 by Western Blot of Immunprecipitate.
An antibody to THAP11 at 1µg/ml staining THAP11 in HeLa whole cell lysate immunoprecipitated using ab113210 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Anti-THAP11 antibody (ab113210)参考文献
has not yet been referenced specifically in any publications.