Anti-THAP11抗体(ab104395)

概述

  • 产品名称Anti-THAP11抗体
    参阅全部 THAP11 一抗
  • 描述
    兔多克隆抗体to THAP11
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Recombinant Fragment
    预测可用于: Rat, Rabbit, Hamster, Cow, Human, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human THAP11.

  • 阳性对照
    • This antibody gave a positive signal in THAP11 recombinant protein as well as the following tissue lysates: Mouse Brain; Mouse Hippocampus.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab104395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

靶标

Anti-THAP11 antibody 图像

  • All lanes : Anti-THAP11 antibody (ab104395) at 1 µg/ml

    Lane 1 : THAP11 Recombinant protein at 0.1 µg
    Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
    Lane 3 : Mouse Hippocampus Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-THAP11 antibody (ab104395)参考文献

ab104395 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 30 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"