Trans Golgi network integral membrane protein 2 antibody
Trans golgi network protein 2 antibody
Trans Golgi network protein TGN51 antibody
Trans-Golgi network integral membrane protein 2 antibody
Trans-Golgi network protein TGN51 antibody
Anti-TGN46 antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)This image is courtesy of an abreview submitted by Dr Armen Petrosyan, Univ of Nebraska.
Immunocytochemistry/ Immunofluorescence analysis of Panc-1 cells labeling TGN46 with ab50595 at 1/100 dilution. Cells were fixed with formaldehyde followed by blocking in 1% Donkey serum in PBST for 1 hour at 22°C. A donkey-anti-Rb Alexa Fluor® 594 was used as the secondary antibody at 1/200 dilution. DAPI nulcear counter stain.
Immunocytochemistry/ Immunofluorescence - TGN46 antibody (ab50595)This image is courtesy of an anonymous Abreview
ab50595 staining TGN46 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.1% Triton X100 and blocked with 2% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA + 0.1% Triton X100) for 2 hours at 25°C. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG (dilution 1/1500) was used as secondary antibody.
Human A549 cells were fixed and permeabilized with methanol followed by acetone fixation. Fixed cells were stained with 2.5 µg/ml ab50595. The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)This image is courtesy of Alessandro Marcello, Int Ctr Genet Engn & Biotechnol, Italy
ab50595 staining human osteosarcoma (143b) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to rabbit diluted 1/500.
Anti-TGN46 antibody (ab50595)参考文献
This product has been referenced in:
Baschieri F et al. Spatial control of Cdc42 signalling by a GM130-RasGRF complex regulates polarity and tumorigenesis. Nat Commun5:4839 (2014).
Read more (PubMed: 25208761) »
Duquet A et al. A novel genome-wide in vivo screen for metastatic suppressors in human colon cancer identifies the positive WNT-TCF pathway modulators TMED3 and SOX12. EMBO Mol Med6:882-901 (2014).
Read more (PubMed: 24920608) »