概述

  • 产品名称Anti-TGN46抗体
    参阅全部 TGN46 一抗
  • 描述
    兔多克隆抗体to TGN46
  • 特异性This antibody does not cross react with TGN46 - the human homologue of TGN46. A Rabbit polyclonal to human TGN46 is available (ab16052).
  • 经测试应用适用于: IHC-P, WB, ICC/IF, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Chinese Hamster
    不与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Mouse TGN46.

    (Peptide available as ab16384.)

  • 阳性对照
    • ab16059 gave a positive result in the following whole cell lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 35 kDa).

Rodent TGN46 is extensively glycosylated and as a result has a mass of 97 kDa on an SDS-PAGE gel.

ICC/IF Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.

靶标

  • 功能May be involved in regulating membrane traffic to and from trans-Golgi network.
  • 组织特异性Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
  • 细胞定位Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • TGN 46 antibody
    • TGN 51 antibody
    • TGN38 antibody
    • TGN38 homolog antibody
    • TGN46 antibody
    • TGN48 antibody
    • TGN51 antibody
    • TGOLN2 antibody
    • TGON2_HUMAN antibody
    • Trans Golgi network integral membrane protein 2 antibody
    • Trans golgi network protein 2 antibody
    • Trans Golgi network protein TGN51 antibody
    • Trans-Golgi network integral membrane protein 2 antibody
    • Trans-Golgi network protein TGN51 antibody
    • TTGN2 antibody
    see all

Anti-TGN46 antibody 图像

  • All lanes : Anti-TGN46 antibody (ab16059) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 35 kDa
    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Rodent TGN46 is extensively glycosylated and as a result has a mass of 97 kDa on an SDS-PAGE gel.

  • ICC/IF image of ab16059 stained mouse MEF1 cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab16059, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of ab16059 staining in mouse e14 whole foetus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16059, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-TGN46 antibody (ab16059)参考文献

This product has been referenced in:
  • Vural A  et al. Activator of G-Protein Signaling 3-Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection. J Immunol 196:846-56 (2016). Read more (PubMed: 26667172) »
  • Oetjen S  et al. Revisiting the neuronal localization and trafficking of CLN3 in juvenile neuronal ceroid lipofuscinosis. J Neurochem 139:456-470 (2016). Read more (PubMed: 27453211) »

See all 12 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic Heart)
Permeabilization Yes - Tween-20
Specification Embryonic Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Rat Cell (Rat liver tissue section)
Specification Rat liver tissue section
Permeabilization Yes - PBS containing 0.25% Triton X-100
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 18 2014


We initially tested ab16059 on human samples and did not detect any cross-reactivity with the human samples. Thus, the antibody should not detect human TGN46, and is tested and guaranteed to detect mouse, rat, and chinese hamster TGN46.

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Chinese Hamster Cell (Chinese Hamster Ovary Cell)
Specification Chinese Hamster Ovary Cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton
Blocking step FCS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (keratinocytes)
Specification keratinocytes
Fixative Paraformaldehyde
Permeabilization Yes - 0.3%Triton in PBS
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"