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Synthetic peptide corresponding to Rat TGN46 aa 1-17.
Our Abpromise guarantee covers the use of ab2809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22456512|
|ICC||1/20 - 1/50.|
This antibody detects an ~38 kDa protein representing recombinant rat TGN38.
|Flow Cyt||1/20 - 1/50.|
Overlay histogram showing CHO cells stained with ab2809 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2809, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in CHO cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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