概述

  • 产品名称Anti-TGFBI抗体
    参阅全部 TGFBI 一抗
  • 描述
    兔多克隆抗体to TGFBI
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: AEPDIMATNG VVHVITNVLQ PPANRPQERG DELADSALEI FKQASAFSRA, corresponding to C terminal amino acids 613-662 of Human TGFBI

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab66957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
  • 组织特异性Highly expressed in the corneal epithelium.
  • 疾病相关Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD) [MIM:121820]; also known as Cogan corneal dystrophy or map-dot-fingerprint type corneal dystrophy. EBMD is a bilateral anterior corneal dystrophy characterized by grayish epithelial fingerprint lines, geographic map-like lines, and dots (or microcysts) on slit-lamp examination. Pathologic studies show abnormal, redundant basement membrane and intraepithelial lacunae filled with cellular debris. Although this disorder usually is not considered to be inherited, families with autosomal dominant inheritance have been identified.
    Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1) [MIM:121900]; also known as corneal dystrophy granular type. Inheritance is autosomal dominant. Corneal dystrophies show progressive opacification of the cornea leading to severe visual handicap.
    Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1) [MIM:122200]. Inheritance is autosomal dominant.
    Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB) [MIM:602082]; also known as corneal dystrophy of Bowman layer type 2 (CDB2).
    Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB) [MIM:608470]; also known as corneal dystrophy of Bowman layer type 1 (CDB1).
    Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A) [MIM:608471]. CDL3A clinically resembles to lattice corneal dystrophy type 3, but differs in that its age of onset is 70 to 90 years. It has an autosomal dominant inheritance pattern.
    Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD) [MIM:607541]. ACD could be considered a variant of granular dystrophy with a significant amyloidogenic tendency. Inheritance is autosomal dominant.
  • 序列相似性Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • 翻译后修饰Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 细胞定位Secreted > extracellular space > extracellular matrix. May be associated both with microfibrils and with the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • RGD containing collagen associated protein antibody
    • AI181842 antibody
    • AI747162 antibody
    • Beta ig antibody
    • Beta ig h3 antibody
    • Beta ig-h3 antibody
    • BGH3_HUMAN antibody
    • Big h3 antibody
    • BIGH3 antibody
    • CDB1 antibody
    • CDG2 antibody
    • CDGG1 antibody
    • CSD antibody
    • CSD1 antibody
    • CSD2 antibody
    • CSD3 antibody
    • EBMD antibody
    • Kerato epithelin antibody
    • Kerato-epithelin antibody
    • LCD1 antibody
    • MGC150270 antibody
    • RGD CAP antibody
    • RGD-CAP antibody
    • RGD-containing collagen-associated protein antibody
    • TGFBI antibody
    • TGFBI transforming growth factor, beta induced, 68kDa antibody
    • Transforming growth factor beta induced protein ig h3 antibody
    • Transforming growth factor-beta-induced protein ig-h3 antibody
    see all

Anti-TGFBI antibody 图像

  • All lanes : Anti-TGFBI antibody (ab66957) at 2.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.
  • Immunofluorescence analysis of Human corneal tissue staining TGFBI with ab66957.

  • Anti-TGFBI antibody (ab66957) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-rabbit IgG at 50,000 - 100,000 dilution

    Observed band size : 46 kDa (why is the actual band size different from the predicted?)

Anti-TGFBI antibody (ab66957)参考文献

This product has been referenced in:
  • Huang AH  et al. Evaluation of the complex transcriptional topography of mesenchymal stem cell chondrogenesis for cartilage tissue engineering. Tissue Eng Part A 16:2699-708 (2010). Read more (PubMed: 20367254) »

See 1 Publication for this product

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