Anti-TGF beta Receptor III抗体(ab10854)

概述

  • 产品名称
    Anti-TGF beta Receptor III抗体
    参阅全部 TGF beta Receptor III 一抗
  • 描述
    山羊多克隆抗体to TGF beta Receptor III
  • 特异性
    Recognizes the extracellular domain of human TGFBR3. By ELISA, the antibody shows no cross-reactivity with other cytokines tested: rhANG, rhAR, rhB7-1, rhB7-2, rmB7-2, rhBTC, rhb-NGF, rhBDNF, rmC10, rhCD4, rhCD8, rhCD28, rhCNTF, rrCNTF, rhEGF, rhENA-78, rhEpo, rhFGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-9, rhG-CSF, rmG-CSF, rhGM-CSF, rhGM-CSF Ralpha, rmGM-CSF, rhGROalpha, rhGRObeta, rhGROgamma, rhHB-EGF, rhHRG-alpha, rhHGF, rhI-309, rhIFN-g, rhIGF-I, rhIGF-I R, rhIGF-II, rhIL-1alpha, rhIL-1 RI, rhIL-1 RII, rmIL-1alpha, rhIL-1beta, rmIL-1beta, rhIL-1ra, rmIL-1ra, rhIL-2, rhIL-2 sRalpha, rhIL-2 sRbeta, IL-2 sRgamma, rmIL-2, rhIL-3, rhIL-3 sRalpha, rmIL-3, rhIL-4, rhIL-4 sR, rmIL-4, rhIL-5, rhIL-5 sRalpha, rhIL-5 sRbeta, rmIL-5, rhIL-6, rhIL-6 sR, rmIL-6, rhIL-7, rhIL-7 R, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, rhIL-10 sR, rmIL-10, rhIL-11, rhIL-12, rmIL-12, rhIL-13, rmIL-13, rhIL-15, rhIP-10, rhJAK-1, rmJAK-1, rmJE, rhLIF, rhLIF R, rmLIF, rhM-CSF, rmM-CSF, rhMCP-1, rhMCP-1 R, rhMCP-2, rhMCP-3, rhMidkine, rhMIP-1alpha, rmMIP-1alpha, rhMIP-1beta, rmMIP-1beta, rmMIP-2, rhNT-3, rhNT-4, rhOSM, rhPD-ECGF, hPDGF, pPDGF, rhPDGF-AA, rhPDGF-AB, rhPDGF-BB, rhPDGF Ralpha, rhPIGF, rhPTN, rhRANTES, rhSCF, rmSCF, rhsgp130, rhSLPI, rhSTAT-1, rmSTAT-4, hTfR, rhTGF-alpha, rhTGF-beta1, rhTGF-beta2, rhTGF-beta3, raTGF-beta5, rhLAP (TGF-beta1), rhLatent TGF-beta1, rhTGF-beta sRII, rhTNF-alpha, rmTNF-alpha, rhTNF-beta, rhsTNF RI, rhsTNF RII, rhTPO, rhVEGF
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human soluble TGFBR3, expressed in NSO cells.

  • 阳性对照
    • Recombinant human TGF beta soluble RIII. Any cells that express the protein should be suitable. The purified form of the protein may also be used to verify detection. Please note that since this protein is soluble, carefull analysis of the culture supernatant should be done.
  • 常规说明
    Endotoxin level is < 10 ng per mg antibody as determined by the LAL method.

性能

应用

Our Abpromise guarantee covers the use of ab10854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.5 - 1 µg/ml.

Use at a concentration of 0.5 - 1 µg/ml to detect a limit of 0.06 ng/well of human TGFBR3.

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 93.5 kDa.

This concentration is determined using 2 ng/lane of human TGFBR3 under non-reducing and reducing conditions

靶标

  • 功能
    Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors.
  • 序列相似性
    Contains 1 ZP domain.
  • 翻译后修饰
    Extensively modified by glycosaminoglycan groups (GAG).
  • 细胞定位
    Secreted. Secreted > extracellular space. Cell membrane. Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Beta glycan antibody
    • Betaglycan antibody
    • Betaglycan proteoglycan antibody
    • BGCAN antibody
    • TGBR3_HUMAN antibody
    • TGF beta receptor type 3 antibody
    • TGF beta receptor type III antibody
    • TGF-beta receptor type 3 antibody
    • TGF-beta receptor type III antibody
    • TGFB R3 antibody
    • TGFBR 3 antibody
    • TGFBR3 antibody
    • TGFR 3 antibody
    • TGFR-3 antibody
    • TGFR3 antibody
    • Transforming growth factor beta receptor III antibody
    • Transforming growth factor beta receptor III betaglycan 300kDa antibody
    • Transforming growth factor beta receptor type 3 antibody
    see all

实验方案

文献

ab10854 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your inquiry. The only option I found was ab28366- Rabbit polyclonal. Immunogen: Recombinant human TGF beta Receptor III protein encoding amino acids 644-850 expressed in E. coli. IHC-P. ab28367 (ready to use version of ab28366). ...

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Application
Western blot
Sample
Human Cell lysate - whole cell (Human Renal Proximal Tubular cells)
Loading amount
10 µg
Specification
Human Renal Proximal Tubular cells
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Robert Jenkins

Verified customer

提交于 May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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