The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
Transcriptional repressor, which probably repress transcription by binding directly the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins. Probably represses transcription via the recruitment of histone deacetylase proteins.
Widely expressed. Highly expressed in heart, kidney and testis. Weakly expressed in brain and prostate.
Belongs to the TALE/TGIF homeobox family. Contains 1 homeobox DNA-binding domain.
The C-terminal part is phosphorylated in response to EGF signaling by the Ras/MAPK pathway.