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A synthetic peptide derived from the C-terminus of the precursor form of human TGF beta 3.
Our Abpromise guarantee covers the use of ab15537 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use at an assay dependent concentration.
The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition.
|IHC-P||1/100. Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Ab15537 demonstrates cytoplasmic staining.|
|IP||Use at an assay dependent concentration.|
The blot was blocked with 5% milk for 1 hour at 21°C and incubated with the primary antibody for 12 hours at 4°C.
Detects a 50 kDa species, which is the presumed TGF-Beta 3 precursor, across all cell lysates and species tested including: Hu, AGMk Ms, Rat, and Hm. Higher molecular weight species are observed in all lysates, which could be glycosylated forms of the precursor. A fast migrating species (~14 kDa) was observed in U2OS cell lysate (Lane 2), which could be the cleaved, active form of TGF-Beta3.