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A synthetic peptide derived from the C-terminus of the precursor form of human TGF beta 3.
Our Abpromise guarantee covers the use of ab15537 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use at an assay dependent concentration. The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition and 25 kDa under nonreducing condition.|
|IHC-P||1/100. Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Ab15537 demonstrates cytoplasmic staining.|
|IP||Use at an assay dependent concentration.|
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