概述

  • 产品名称Anti-TGF beta 2抗体
    参阅全部 TGF beta 2 一抗
  • 描述
    兔多克隆抗体to TGF beta 2
  • 特异性This antibody is raised against the latency associated peptide (LAP) region of TGFB2, so it will not recognize mature TGFB2.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Cow, Dog, Pig, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TGF beta 2.

  • 阳性对照
    • WB: MCF7 whole cell lysate and human breast, human adipose, rat kidney and mouse kidney tissue lysates. ICC/IF: HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab113670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-TGF beta 2 antibody 图像

  • ICC/IF image of ab113670 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab113670, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-TGF beta 2 antibody (ab113670) at 1 µg/ml

    Lane 1 : Human breast tissue lysate - total protein (ab30090)
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : Kidney (Mouse) Tissue Lysate
    Lane 4 : Kidney (Rat) Tissue Lysate
    Lane 5 : Human adipose normal tissue lysate - total protein (ab28980)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds

Anti-TGF beta 2 antibody (ab113670)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab113670.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"