Anti-TGF beta 1抗体(ab9758)
Key features and details
- Rabbit polyclonal to TGF beta 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TGF beta 1抗体
参阅全部 TGF beta 1 一抗 -
描述
兔多克隆抗体to TGF beta 1 -
宿主
Rabbit -
特异性
Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, Pig不与反应: Dog -
免疫原
Synthetic peptide corresponding to Human TGF beta 1.
Sequence:FSSTEKN-C
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (3) |
1/100 - 1/500.
In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
说明 |
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WB
1/100 - 1/500. In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB. |
靶标
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功能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
组织特异性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
疾病相关
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
序列相似性
Belongs to the TGF-beta family. -
翻译后修饰
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 282089 Cow
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P18341 Cow
- SwissProt: P01137 Human
- Unigene: 645227 Human
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别名
- Cartilage-inducing factor antibody
- CED antibody
- Differentiation inhibiting factor antibody
see all
图片
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All lanes : Anti-TGF beta 1 antibody (ab9758) at 1/200 dilution
Lane 1 : Reduced human recombinant TGF beta 1
Lane 2 : Non-reduced human recombinant TGF beta 1
Lysates/proteins at 0.25 µg per lane.
Secondary
All lanes : Peroxidase-conjugated anti-rabbit IgG at 1/40000 dilution
Observed band size: 12,25 kDa why is the actual band size different from the predicted?Incubated with the primary antibody overnight at 4°C.
Incubated with the secondary antibody for 30 min at room temperature.
数据表及文件
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SDS download
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Datasheet download
文献 (59)
ab9758 被引用在 59 文献中.
- Qu XF et al. Pyrroloquinoline quinone ameliorates diabetic cardiomyopathy by inhibiting the pyroptosis signaling pathway in C57BL/6 mice and AC16 cells. Eur J Nutr 61:1823-1836 (2022). PubMed: 34997266
- Popek M et al. The Effect of TGF-β1 Reduced Functionality on the Expression of Selected Synaptic Proteins and Electrophysiological Parameters: Implications of Changes Observed in Acute Hepatic Encephalopathy. Int J Mol Sci 23:N/A (2022). PubMed: 35163004
- Fu X et al. Long non-coding RNA NCK1-AS1 is overexpressed in esophageal squamous cell carcinoma and predicts survival. Bioengineered 13:8302-8310 (2022). PubMed: 35311444
- Zhuang X et al. Ocular Manifestations in a Chinese Pedigree of Familial Amyloidotic Polyneuropathy Carrying the Transthyretin Mutation c.401A>G (p.Tyr134Cys). Genes (Basel) 13:N/A (2022). PubMed: 35627273
- Moyano A et al. PD-L1 is upregulated in CD163+ tonsillar macrophages from children undergoing EBV primary infection. Front Immunol 13:940910 (2022). PubMed: 36451810