概述

  • 产品名称
    Anti-TGF beta 1抗体
    参阅全部 TGF beta 1 一抗
  • 描述
    兔多克隆抗体to TGF beta 1
  • 特异性
    Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
  • 经测试应用
    适用于: Flow Cyt, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, Pig不与反应: Dog
  • 免疫原

    Synthetic peptide corresponding to Human TGF beta 1.
    Sequence:

    FSSTEKN-C

  • 阳性对照
    • WB: Recombinant human TGF beta 1.

性能

应用

Our Abpromise guarantee covers the use of ab9758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 25421510
IHC-P Use at an assay dependent concentration. PubMed: 25207642
WB 1/100 - 1/500.

In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

靶标

  • 功能
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 组织特异性
    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 疾病相关
    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 序列相似性
    Belongs to the TGF-beta family.
  • 翻译后修饰
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

图片

  • All lanes : Anti-TGF beta 1 antibody (ab9758) at 1/200 dilution

    Lane 1 : Reduced recombinant TGF beta 1
    Lane 2 : Non-reduced recombinant TGF beta 1

    Lysates/proteins at 0.25 µg per lane.

    Secondary
    Peroxidase-conjugated anti-rabbit IgG at 1/40000 dilution

    Observed band size : 12,25 kDa (why is the actual band size different from the predicted?)

    Incubated with the primary antibody overnight at 4°C.

    Incubated with the secondary antibody for 30 min at room temperature.

  • Five micron sections of paraffin embedded peritoneum tissue were mounted onto electrostatically charged microscope slides and dewaxed in xylene (2×5min) and rehydrated. Antigens were retrieval by pressure-cooking slides in 10 mM Tris 1 mM EDTA pH 9, for 5 mins. Slides were washed in H2O before incubation with 3% hydrogen peroxide for 30 min. Slides were blocked then incubated with ab9758 diluted 1/1000 or rabbit IgG in blocking buffer overnight at 4°C. Slides were washed in TBST20 and incubated for 30 min with secondary antibody and washed again before incubation with 3, 3′-diaminobenzidine for 5 min. Slides were then counterstained with hematoxylin, dehydrated and visualized by light microscopy, using an Olympus Provis microscope equipped with a Kodak DCS330 camera.

文献

This product has been referenced in:
  • Jin Y  et al. Upregulation of long non-coding RNA PlncRNA-1 promotes proliferation and induces epithelial-mesenchymal transition in prostate cancer. Oncotarget 8:26090-26099 (2017). Read more (PubMed: 28212533) »
  • Fong G  et al. The effects of substance P and acetylcholine on human tenocyte proliferation converge mechanistically via TGF-ß1. PLoS One 12:e0174101 (2017). Read more (PubMed: 28301610) »

See all 15 Publications for this product

客户评价及客户问答

Thank you for contacting Abcam.

We spoke earlier regarding the expected western blot band size associated with TGF beta-1, detected by antibody ab64715. As we discussed, the molecular weight of the TGF beta-1 protein is about 45 kDa, which is ...

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Thank you for contacting us.

We do not have mouse monoclonal antibodies to the target you are requested. However we do have following antibodies available;

ab9758; http://www.abcam.com/TGF-beta-1-antibody-ab9758.html

ab46780;...

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Thank for answering the questions of the Abcam "post complaint survey".

I understand that you have not used the replacement kit yet.
I would like to remind you that this free of charge replacement kit is also covered by our Abpro...

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Thank you for your feedback! We are always happy to help, and if you have any further questions please let me know.

Gracias por tomarte la molestia de responder nuestro cuestionario.

He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría hacer para intentar mejorar los resultados del anticuerpo.

Es posible que no...

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Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.
...

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Thank you for confirming those details. I have now arranged for the chicken polyclonal we discussed to be sent out to you at the address specified. The new order number is xxxxx(purchase order number xxxxxx). This should be with you tomorrow.

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Thank you for getting back to me with that information and sorry for the delay in getting back to you.

From the blot you have shared with me, it does indeed look like you are detecting the protein as expected, especially in the human thromboc...

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Thank you for getting back to me with how you have been getting on with the anti-TGF beta 1 antibody(ab9758).

I am glad to hear that you thinkyou have been detecting the expected protein. If you would like help in optimising the experiment fu...

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Thank you very much for your interest in ab9758.

Unfortunately, this antibody has not been tested in opossum and the immunogen also does not show a significant homology to opossum according to NCBI Blast.

But rat shows a 100% seque...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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