概述

  • 产品名称Anti-TGF beta 1抗体
    参阅全部 TGF beta 1 一抗
  • 描述
    兔多克隆抗体to TGF beta 1
  • 特异性Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
  • 经测试应用适用于: WB, IHC-FrFl, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Human TGF beta 1. Surrounding amino acid 374.

  • 阳性对照
    • RAW264.7 cell lysate; Mouse muscle lysate

性能

应用

Our Abpromise guarantee covers the use of ab92486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 44 kDa.

Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

IHC-FrFl Use at an assay dependent concentration. PubMed: 24647450
IHC-P Use a concentration of 10 - 20 µg/ml.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
ICC/IF 1/100.

靶标

  • 功能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 组织特异性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 疾病相关Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 序列相似性Belongs to the TGF-beta family.
  • 翻译后修饰Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CED antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    see all

Anti-TGF beta 1 antibody 图像

  • All lanes : Anti-TGF beta 1 antibody (ab92486) at 4 µg/ml

    Lane 1 : Mouse muscle lysate at 100 µg
    Lane 2 : TGF beta 1 Human recombinant protein at 0.05 µg


    Predicted band size : 44 kDa

    Blocked using 5% milk TTBS.



  • Predicted band size : 44 kDa

    Western blot of mouse 3T3 cell lysate with ab92486 at concentration 4 ug/ml.

  • ab92486 staining TGF beta 1 in Mouse intastine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 24 hours at 4°C. An undiluted HRP-conjugated Human anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-TGF beta 1 antibody (ab92486) at 0.5 µg/ml + RAW264.7 cell lysate 20-40 ug

    Predicted band size : 44 kDa
  • ab92486 staining TGF beta 1 in Human retina membrane by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/400 in PBS + 1% Triton X-100 + 2% goat serum) for 24 hours at 4°C. An HRP-conjugated Human polyclonal was used as the secondary antibody.

    See Abreview

Anti-TGF beta 1 antibody (ab92486)参考文献

This product has been referenced in:
  • Zhang G  et al. Ameliorative effects of Xue-Fu-Zhu-Yu decoction, Tian-Ma-Gou-Teng-Yin and Wen-Dan decoction on myocardial fibrosis in a hypertensive rat mode. BMC Complement Altern Med 16:56 (2016). WB ; Rat . Read more (PubMed: 26852136) »
  • Fang L  et al. Jumonji AT-rich interactive domain 1B overexpression is associated with the development and progression of glioma. Int J Mol Med 38:172-82 (2016). Human . Read more (PubMed: 27246838) »

See all 32 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Mouse Tissue (Brain)
Specification Brain
Username

Dr. Kate Hawkins

Verified customer

提交于 Nov 23 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (skin)
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 May 26 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample Rabbit Tissue sections (intestinum)
Specification intestinum
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 01 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample Pig Tissue sections (liver)
Specification liver
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 13 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Rat Tissue sections (brain)
Specification brain
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (1618K, Testis cancer cells)
Specification 1618K, Testis cancer cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Ms. Beate Thode

Verified customer

提交于 Feb 22 2013

Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (intestine mouse)
Specification intestine mouse
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citratebuffer
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Andrea Dannullis

Verified customer

提交于 Aug 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (membrane peeled by retina)
Specification membrane peeled by retina
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Ms. Andrea Dannullis

Verified customer

提交于 Jul 16 2012

Thank you for your inquiry.

Yes, this antibody recognizes the precursor, inactive and active forms of TGF-beta 1, at-least by western blotting.

Here are some references which provide more info about its use:

Danussi, C. et...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"