Anti-TGF beta 1抗体(ab46780)

概述

  • 产品名称Anti-TGF beta 1抗体
    参阅全部 TGF beta 1 一抗
  • 描述
    鸡多克隆抗体to TGF beta 1
  • 特异性Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
    预测可用于: a wide range of other species
  • 免疫原

    Synthetic peptide:

    RKPKVEQLSNMIVRS

    , corresponding to amino acids 372-386 of Human and Mouse TGF beta 1.

  • 阳性对照

性能

应用

Our Abpromise guarantee covers the use of ab46780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
ELISA
  • 应用说明ELISA: 1/2000 - 1/5000.
    WB: 1/2000 - 1/5000. Predicted molecular weight: 47 kDa for the unprocessed precursor; 25 kDa for the mature protein; 13KDa for degraded or cleaved protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • 组织特异性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • 疾病相关Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • 序列相似性Belongs to the TGF-beta family.
    • 翻译后修饰Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CED antibody
      • DPD1 antibody
      • LAP antibody
      • Latency-associated peptide antibody
      • Prepro transforming growth factor beta 1 antibody
      • TGF beta 1 antibody
      • TGF beta antibody
      • TGF beta 1 protein antibody
      • TGF-beta 1 protein antibody
      • TGF-beta-1 antibody
      • TGF-beta-5 antibody
      • TGF-beta1 antibody
      • TGFB antibody
      • Tgfb-1 antibody
      • tgfb1 antibody
      • TGFB1_HUMAN antibody
      • TGFbeta antibody
      • TGFbeta1 antibody
      • Transforming Growth Factor b1 antibody
      • Transforming Growth Factor beta 1 antibody
      • Transforming growth factor beta 1a antibody
      • transforming growth factor beta-1 antibody
      • transforming growth factor, beta 1 antibody
      see all

    Anti-TGF beta 1 antibody 图像

    • ICC/IF image of ab46780 stained T24/83 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46780 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- chicken (ab150173) IgY (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • All lanes : Anti-TGF beta 1 antibody (ab46780) at 1/2000 dilution (ab50036, 0.1ug)

      Lane 1 : As above
      Lane 2 : As above

      Secondary
      Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

    Anti-TGF beta 1 antibody (ab46780)参考文献

    ab46780 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    We do not have mouse monoclonal antibodies to the target you are requested. However we do have following antibodies available;

    ab9758; http://www.abcam.com/TGF-beta-1-antibody-ab9758.html

    ab46780;...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"