Anti-TGF beta 1抗体[9016.2] (ab10517)

概述

  • 产品名称Anti-TGF beta 1抗体[9016.2]
    参阅全部 TGF beta 1 一抗
  • 描述
    小鼠单克隆抗体[9016.2] to TGF beta 1
  • 特异性By ELISA, the antibody shows less than 5% crossreactivity with TGF beta 1.2 and less than 1% cross-reactivity with TGF beta 2, TGF beta 3 and TGF beta 5. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
  • 经测试应用适用于: ELISA, Inhibition Assay, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human) expressed in CHO cells.

  • 阳性对照

性能

应用

Our Abpromise guarantee covers the use of ab10517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
Inhibition Assay
WB
  • 应用说明WB: Use at a concentration of 1 - 2 µg/ml. Determined for 20 ng recombinant human TGF Beta 1 under non-reducing conditions. Predicted molecular weight: 47 kDa for the unprocessed precursor; 25 kDa for the mature protein.
    ELISA: Use at a concentration of 2 - 4 µg/ml. Determined for 9 pg/ml of recombinant human TGF beta 1.
    Inhib: Use at a concentration of 0.3 - 1 µg/ml.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • 组织特异性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • 疾病相关Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • 序列相似性Belongs to the TGF-beta family.
    • 翻译后修饰Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CED antibody
      • DPD1 antibody
      • LAP antibody
      • Latency-associated peptide antibody
      • Prepro transforming growth factor beta 1 antibody
      • TGF beta 1 antibody
      • TGF beta antibody
      • TGF beta 1 protein antibody
      • TGF-beta 1 protein antibody
      • TGF-beta-1 antibody
      • TGF-beta-5 antibody
      • TGF-beta1 antibody
      • TGFB antibody
      • Tgfb-1 antibody
      • tgfb1 antibody
      • TGFB1_HUMAN antibody
      • TGFbeta antibody
      • TGFbeta1 antibody
      • Transforming Growth Factor b1 antibody
      • Transforming Growth Factor beta 1 antibody
      • Transforming growth factor beta 1a antibody
      • transforming growth factor beta-1 antibody
      • transforming growth factor, beta 1 antibody
      see all

    Anti-TGF beta 1 antibody [9016.2] (ab10517)参考文献

    This product has been referenced in:
    • Soller EC  et al. Common features of optimal collagen scaffolds that disrupt wound contraction and enhance regeneration both in peripheral nerves and in skin. Biomaterials 33:4783-91 (2012). WB ; Rat . Read more (PubMed: 22483241) »
    • Wang L  et al. Concentration-dependent effects of transforming growth factor ß1 on corneal wound healing. Mol Vis 17:2835-46 (2011). Inhibition Assay ; Human . Read more (PubMed: 22128231) »

    See all 4 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - whole (Peripheral nerve wound)
    Loading amount 15 µg
    Specification Peripheral nerve wound
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Dec 11 2008

    Thank you for your enquiry. In general, a neutralizing antibody is defined to be an antibody that prevents a virus from infecting a cell, usually by blocking receptors on the cells or virus. A blocking antibody is defined to be an antibody that combine...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"