特异性By ELISA, the antibody shows less than 5% crossreactivity with TGF beta 1.2 and less than 1% cross-reactivity with TGF beta 2, TGF beta 3 and TGF beta 5. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: Use at a concentration of 1 - 2 µg/ml. Determined for 20 ng recombinant human TGF Beta 1 under non-reducing conditions. Predicted molecular weight: 47 kDa for the unprocessed precursor; 25 kDa for the mature protein.
ELISA: Use at a concentration of 2 - 4 µg/ml. Determined for 9 pg/ml of recombinant human TGF beta 1.
Inhib: Use at a concentration of 0.3 - 1 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
组织特异性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
疾病相关Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
序列相似性Belongs to the TGF-beta family.
翻译后修饰Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
细胞定位Secreted > extracellular space > extracellular matrix.
Soller EC et al. Common features of optimal collagen scaffolds that disrupt wound contraction and enhance regeneration both in peripheral nerves and in skin. Biomaterials33:4783-91 (2012).
Read more (PubMed: 22483241) »
Wang L et al. Concentration-dependent effects of transforming growth factor ß1 on corneal wound healing. Mol Vis17:2835-46 (2011).
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