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Our Abpromise guarantee covers the use of ab9585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Can be blocked with Recombinant TGF alpha protein (ab9587).|
|Neutralising||Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 µg/ml of this antibody is required.
|Sandwich ELISA||Use at an assay dependent concentration.
To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. The antibody, in conjunction with biotinylated Anti-Human TGF-α ab83159 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α.
|ELISA||Use a concentration of 0.5 µg/ml.|
|IHC-P||Use a concentration of 0.25 µg/ml.|