概述

  • 产品名称Anti-TGF alpha抗体
    参阅全部 TGF alpha 一抗
  • 描述
    兔多克隆抗体to TGF alpha
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Rabbit, Cow, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human TGF alpha aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P01135

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissues: Human skin melanoma and Rat normal kidney.

性能

应用

Our Abpromise guarantee covers the use of ab112030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 - 5 µg/ml.

靶标

Anti-TGF alpha antibody 图像

  • IHC image of TGF alpha staining in Rat normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112030, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of TGF alpha staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112030, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-TGF alpha antibody (ab112030)参考文献

ab112030 has not yet been referenced specifically in any publications.

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