概述

  • 产品名称Anti-TFPI2抗体
    参阅全部 TFPI2 一抗
  • 描述
    兔多克隆抗体to TFPI2
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chicken
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 72-121 (

    NANNFYTWEACDDACWRIEKVPKVCRLQVSVDDQCEGSTEKYFFNLSSMT

    ) of Human TFPI2 (NP_006519)

  • 阳性对照
    • MCF7 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

靶标

  • 功能May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.
  • 组织特异性Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy.
  • 序列相似性Contains 3 BPTI/Kunitz inhibitor domains.
  • 结构域This inhibitor contains three inhibitory domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Placental protein 5 antibody
    • PP5 antibody
    • REF1 antibody
    • Retinal pigment epithelium cell factor 1 antibody
    • TFPI 2 antibody
    • TFPI-2 antibody
    • TFPI2 antibody
    • TFPI2 PEN antibody
    • TFPI2_HUMAN antibody
    • Tissue factor pathway inhibitor 2 antibody
    see all

Anti-TFPI2 antibody 图像

  • Anti-TFPI2 antibody (ab83470) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 27 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of Human Macrophage Cells staining TFPI2 (green/yellow) with ab83470 at 1/200 dilution. A FITC-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

Anti-TFPI2 antibody (ab83470)参考文献

ab83470 has not yet been referenced specifically in any publications.

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