概述

  • 产品名称Anti-TFPI抗体
    参阅全部 TFPI 一抗
  • 描述
    山羊多克隆抗体to TFPI
  • 经测试应用适用于: WB, Electron Microscopymore details
  • 种属反应性
    与反应: Human
    预测可用于: Rhesus monkey
  • 免疫原

    Synthetic peptide: VKIAYEEIFVKNM, corresponding to C terminal amino acids 292-304 of Human TFPI.

  • 阳性对照
    • HeLa lysate
  • 常规说明Principal Names - TFPI: tissue factor pathway inhibitor; Lipoprotein-associated coagulation inhibitor; LACI Official Gene Symbol - TFPI GenBank Accession Number – NP_006278 LocusLink ID - 7035


    A protease inhibitor that inhibits fibrin clot formation.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明A protease inhibitor that inhibits fibrin clot formation.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab9881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Can be blocked with Human TFPI peptide (ab23168).
Electron Microscopy Use at an assay dependent dilution. PubMed: 20592020

靶标

  • 功能Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 组织特异性Mostly in endothelial cells.
  • 序列相似性Contains 3 BPTI/Kunitz inhibitor domains.
  • 结构域This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anti convertin antibody
    • EPI antibody
    • Extrinsic pathway inhibitor antibody
    • LACI antibody
    • Lipoprotein associated coagulation inhibitor antibody
    • Lipoprotein-associated coagulation inhibitor antibody
    • TFI antibody
    • TFPI 1 antibody
    • TFPI antibody
    • TFPI1 antibody
    • TFPI1_HUMAN antibody
    • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) antibody
    • Tissue factor pathway inhibitor antibody
    see all

Anti-TFPI antibody (ab9881)参考文献

This product has been referenced in:
  • Papareddy P  et al. C-terminal Peptides of Tissue Factor Pathway Inhibitor Are Novel Host Defense Molecules. J Biol Chem 285:28387-98 (2010). Electron Microscopy . Read more (PubMed: 20592020) »

See 1 Publication for this product

Product Wall

Thank you for your reply.
Thank you for trying some of the tips. Please do let me know what the results are once you have them. I'm curious myself, as well as want to ensure the antibodies are working as expected.

Should it turn out that...

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Thank you for your reply and the additional information.

I have a couple more questions as well as a few suggestions. I hope you don't mind.

1) Is the rTFPI that you were showing on the left hand side glycosylated or not?
2) ...

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Thank you for contacting us.

Antibody ab9881, detects only the alpha isoform.
Antibody ab66544, would detect both isoforms if they are present
For antibody ab48648, we do not know where the epitope is, thus we cannot predict if eit...

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Thank you for your enquiry. You can use reducing Western blotting conditions (if non-reducing conditions are required, we would state this information on the online datasheet). We would recommend blocking with either 5% w/v nonfat dry milk or 5% w/v BS...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in FACS. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in r...

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This is a new antibody that has only yet been tested on human.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"