Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TFIP11 with ab91520 at 1/200 (1µg/ml). Detection: DAB.
Western blot - TFIP11 antibody (ab91520)
All lanes : Anti-TFIP11 antibody (ab91520) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 30 seconds
Immunoprecipitation - TFIP11 antibody (ab91520)
Ab91520 at 1 µg/ml detecting TFIP11 by WB following IP. Lane 1; ab91520 at 3 µg/mg lysate precipitating TFIP11 from HeLa whole cell lysate. Lane 2; IP using an antibody to a downstream epitope of TFIP11. Lane 3; TFIP11 was not precipitated using control IgG. In each case 1 mg of whole cell lysate from HeLa cells was used for IP and 20% of IP was loaded.
Detection utilised ECL with a 10 second exposure.