概述

  • 产品名称
  • 描述
    兔多克隆抗体to TFIP11
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human TFIP11 (NP_036275.1)

  • 阳性对照
    • HeLa whole cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix. May also be involved in pre-mRNA splicing.
  • 序列相似性
    Belongs to the TFP11/STIP family.
    Contains 1 G-patch domain.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BK445C9.6 antibody
    • DKFZP434B194 antibody
    • FLJ22086 antibody
    • HNtr1 antibody
    • NTR1 antibody
    • NTR1, S. cerevisiae, homolog of antibody
    • OTTHUMP00000198650 antibody
    • OTTHUMP00000198850 antibody
    • OTTHUMP00000198851 antibody
    • OTTHUMP00000198852 antibody
    • Septin and tuftelin-interacting protein 1 antibody
    • Spp382 antibody
    • STIP antibody
    • STIP-1 antibody
    • TFIP11 antibody
    • TFP11_HUMAN antibody
    • TIP39 antibody
    • Tuftelin interacting protein 11 antibody
    • Tuftelin-interacting protein 11 antibody
    • Tuftelin-interacting protein 11, 39-KD antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TFIP11 with ab91520 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TFIP11 antibody (ab91520) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 97 kDa


    Exposure time : 30 seconds
  • Ab91520 at 1 µg/ml detecting TFIP11 by WB following IP. Lane 1; ab91520 at 3 µg/mg lysate precipitating TFIP11 from HeLa whole cell lysate. Lane 2; IP using an antibody to a downstream epitope of TFIP11. Lane 3; TFIP11 was not precipitated using control IgG. In each case 1 mg of whole cell lysate from HeLa cells was used for IP and 20% of IP was loaded.
    Detection utilised ECL with a 10 second exposure.

文献

ab91520 has not yet been referenced specifically in any publications.

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