Anti-TFIIS抗体(ab49352)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to TFIIS
  • 特异性
    This antibody recognizes TFIIS
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A region within synthetic peptide: MPITLHLLQS TRVGMSVNAL RKQSSDEEVI ALAKSLIKSW KKLLDASDAK, corresponding to N terminal amino acids 37-86 of Human TFIIS

  • 阳性对照
    • Fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab49352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能
    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • 组织特异性
    Testis and ovary specific.
  • 序列相似性
    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Tcea2 antibody
    • TCEA2_HUMAN antibody
    • Testis specific S II antibody
    • Testis-specific S-II antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 2 antibody
    • Transcription elongation factor A 2 antibody
    • Transcription elongation factor A protein 2 antibody
    • Transcription elongation factor S II protein 2 antibody
    • Transcription elongation factor S-II protein 2 antibody
    • Transcription elongation factor TFIIS.1 antibody
    • Transcription elongation factor TFIIS.l antibody
    see all

图片

  • ab49352 staining TFIIS in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1 x PBS) for 3 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-TFIIS antibody (ab49352) at 1.25 µg/ml + Fetal brain lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Anti-TFIIS antibody (ab49352) at 1/1000 dilution + Brain Tissue Lysate from 3 week old mice at 30 µg/ml

    Secondary
    Goat anti-rabbit HRP-congugated polyclonal antibody at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

ab49352 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Cell line)
Loading amount
20 µg
Specification
Cell line
Gel Running Conditions
Reduced Denaturing (12 % SDS PAGE gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Poonam Singh

Verified customer

提交于 May 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain three weeks old mice)
Loading amount
30 µg
Specification
Brain three weeks old mice
Gel Running Conditions
Reduced Denaturing (12 % SDS gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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