A region within synthetic peptide: MPITLHLLQS TRVGMSVNAL RKQSSDEEVI ALAKSLIKSW KKLLDASDAK, corresponding to N terminal amino acids 37-86 of Human TFIIS
Our Abpromise guarantee covers the use of ab49352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/200. | |
WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
ELISA | Use at an assay dependent concentration. ELISA titre using peptide based assay: 1:312500. |
ab49352 staining TFIIS in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1 x PBS) for 3 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
ab49352 has not yet been referenced specifically in any publications.
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