TFIIS抗体(ab49352)| Abcam中国

产品名称

Anti-TFIIS抗体
参阅全部 TFIIS 一抗
描述

兔多克隆抗体to TFIIS
宿主

Rabbit
特异性

This antibody recognizes TFIIS
经测试应用

适用于: ICC/IF, WB, ELISAmore details
种属反应性

与反应: Mouse, Human
免疫原

A region within synthetic peptide: MPITLHLLQS TRVGMSVNAL RKQSSDEEVI ALAKSLIKSW KKLLDASDAK, corresponding to N terminal amino acids 37-86 of Human TFIIS

Run BLAST with Run BLAST with
阳性对照
- Fetal brain lysate

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液

Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度

Protein A purified
克隆

多克隆
同种型

IgG
研究领域

Our Abpromise guarantee covers the use of ab49352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Ab评论	说明
ICC/IF		1/200.
WB		Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA		Use at an assay dependent concentration. ELISA titre using peptide based assay: 1:312500.

功能

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
组织特异性

Testis and ovary specific.
序列相似性

Belongs to the TFS-II family.
Contains 1 TFIIS central domain.
Contains 1 TFIIS N-terminal domain.
Contains 1 TFIIS-type zinc finger.
细胞定位

Nucleus.
Target information above from: UniProt accession Q15560 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 6919 Human
- Entrez Gene: 21400 Mouse
- Omim: 604784 Human
- SwissProt: Q15560 Human
- SwissProt: Q9QVN7 Mouse
- Unigene: 505004 Human
- Unigene: 24245 Mouse
别名
- Tcea2 antibody
- TCEA2_HUMAN antibody
- Testis specific S II antibody
- Testis-specific S-II antibody
- TFIIS antibody
- Transcription elongation factor A (SII) 2 antibody
- Transcription elongation factor A 2 antibody
- Transcription elongation factor A protein 2 antibody
- Transcription elongation factor S II protein 2 antibody
- Transcription elongation factor S-II protein 2 antibody
- Transcription elongation factor TFIIS.1 antibody
- Transcription elongation factor TFIIS.l antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-TFIIS antibody (ab49352)This image is courtesy of an anonymous Abreview

ab49352 staining TFIIS in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1 x PBS) for 3 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
See Abreview
Western blot - Anti-TFIIS antibody (ab49352)

Anti-TFIIS antibody (ab49352) at 1.25 µg/ml + Fetal brain lysate at 10 µg with 5% skim milk in PBS.

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 34 kDa
Observed band size: 28 kDa (why is the actual band size different from the predicted?)

Gel concentration: 12%
Western blot - Anti-TFIIS antibody (ab49352)This image is courtesy of an anonymous Abreview

Anti-TFIIS antibody (ab49352) at 1/1000 dilution + Brain Tissue Lysate from 3 week old mice at 30 µg/ml

Secondary
Goat anti-rabbit HRP-congugated polyclonal antibody at 1/1000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 34 kDa

Exposure time: 10 seconds
See Abreview

ab49352 has not yet been referenced specifically in any publications.

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Anti-TFIIS抗体(ab49352)