Anti-TFIIS抗体(ab49352)

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概述

  • 产品名称
    Anti-TFIIS抗体
    参阅全部 TFIIS 一抗
  • 描述
    兔多克隆抗体to TFIIS
  • 特异性
    This antibody recognizes TFIIS
  • 经测试应用
    适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A region within synthetic peptide: MPITLHLLQS TRVGMSVNAL RKQSSDEEVI ALAKSLIKSW KKLLDASDAK, corresponding to N terminal amino acids 37-86 of Human TFIIS

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab49352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能
    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • 组织特异性
    Testis and ovary specific.
  • 序列相似性
    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Tcea2 antibody
      • TCEA2_HUMAN antibody
      • Testis specific S II antibody
      • Testis-specific S-II antibody
      • TFIIS antibody
      • Transcription elongation factor A (SII) 2 antibody
      • Transcription elongation factor A 2 antibody
      • Transcription elongation factor A protein 2 antibody
      • Transcription elongation factor S II protein 2 antibody
      • Transcription elongation factor S-II protein 2 antibody
      • Transcription elongation factor TFIIS.1 antibody
      • Transcription elongation factor TFIIS.l antibody
      see all

    Anti-TFIIS antibody 图像

    • Immunocytochemistry/ Immunofluorescence - Anti-TFIIS antibody (ab49352)
      Immunocytochemistry/ Immunofluorescence - Anti-TFIIS antibody (ab49352)This image is courtesy of an anonymous Abreview

      ab49352 staining TFIIS in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1 x PBS) for 3 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • Western blot - TFIIS antibody (ab49352)
      Western blot - TFIIS antibody (ab49352)
      Anti-TFIIS antibody (ab49352) at 1.25 µg/ml + Fetal brain lysate at 10 µg with 5% skim milk in PBS.

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size : 34 kDa
      Observed band size : 28 kDa (why is the actual band size different from the predicted?)
      Gel concentration: 12%
    • Western blot - Anti-TFIIS antibody (ab49352)
      Western blot - Anti-TFIIS antibody (ab49352)This image is courtesy of an anonymous Abreview
      Anti-TFIIS antibody (ab49352) at 1/1000 dilution + Brain Tissue Lysate from 3 week old mice at 30 µg/ml

      Secondary
      Goat anti-rabbit HRP-congugated polyclonal antibody at 1/1000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 34 kDa


      Exposure time : 10 seconds

      This image is courtesy of an anonymous Abreview

      See Abreview

    Anti-TFIIS antibody (ab49352)参考文献

    ab49352 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab49352？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-TFIIS antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cells)
    Specification
    HeLa cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 1% Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 May 10 2013

    Western blot abreview for Anti-TFIIS antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cell line)
    Loading amount
    20 µg
    Specification
    Cell line
    Gel Running Conditions
    Reduced Denaturing (12 % SDS PAGE gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Ms. Poonam Singh

    Verified customer

    提交于 May 03 2013

    Western blot abreview for Anti-TFIIS antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain three weeks old mice)
    Loading amount
    30 µg
    Specification
    Brain three weeks old mice
    Gel Running Conditions
    Reduced Denaturing (12 % SDS gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Feb 08 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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