概述
- 产品名称Anti-TFIIS抗体
参阅全部 TFIIS 一抗 - 描述兔多克隆抗体to TFIIS
- 特异性This antibody recognizes TFIIS
- 经测试应用适用于: ICC/IF, WB, ELISAmore details
- 种属反应性与反应: Mouse, Human
- 免疫原
A region within synthetic peptide: MPITLHLLQS TRVGMSVNAL RKQSSDEEVI ALAKSLIKSW KKLLDASDAK, corresponding to N terminal amino acids 37-86 of Human TFIIS
- 阳性对照
- Fetal brain lysate
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - 纯度Protein A purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab49352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/200. | |
| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | Use at an assay dependent concentration. ELISA titre using peptide based assay: 1:312500. |
靶标
- 功能Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
- 组织特异性Testis and ovary specific.
- 序列相似性Belongs to the TFS-II family.
Contains 1 TFIIS central domain.
Contains 1 TFIIS N-terminal domain.
Contains 1 TFIIS-type zinc finger. - 细胞定位Nucleus.
- Information by UniProt
-
数据库链接
- Entrez Gene: 6919 Human
- Entrez Gene: 21400 Mouse
- Omim: 604784 Human
- SwissProt: Q15560 Human
- SwissProt: Q9QVN7 Mouse
- Unigene: 505004 Human
- Unigene: 24245 Mouse
-
别名
- Tcea2 antibody
- TCEA2_HUMAN antibody
- Testis specific S II antibody
see all
Anti-TFIIS antibody 图像
-
Immunocytochemistry/ Immunofluorescence - Anti-TFIIS antibody (ab49352)This image is courtesy of an anonymous Abreviewab49352 staining TFIIS in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1 x PBS) for 3 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
-
Western blot - TFIIS antibody (ab49352)Anti-TFIIS antibody (ab49352) at 1.25 µg/ml + Fetal brain lysate at 10 µg with 5% skim milk in PBS.
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 34 kDa
Observed band size : 28 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12% -
Western blot - Anti-TFIIS antibody (ab49352)This image is courtesy of an anonymous AbreviewAnti-TFIIS antibody (ab49352) at 1/1000 dilution + Brain Tissue Lysate from 3 week old mice at 30 µg/ml
Secondary
Goat anti-rabbit HRP-congugated polyclonal antibody at 1/1000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 34 kDa
Exposure time : 10 secondsThis image is courtesy of an anonymous Abreview
Anti-TFIIS antibody (ab49352)参考文献
ab49352 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"