Anti-TFIIIC / GTF3C1抗体(ab75251)

概述

  • 产品名称Anti-TFIIIC / GTF3C1抗体
    参阅全部 TFIIIC / GTF3C1 一抗
  • 描述
    兔多克隆抗体to TFIIIC / GTF3C1
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
    预测可用于: Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 2059 and 2109 of human TFIIIC/ GTF3C1 protein (NP_001511.2)

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab75251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 238 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 相关性The transcription factor IIIC (TFIIIC) is a multi-subunit DNA-binding factor required for promoter recognition and TFIIIB assembly on tRNA genes transcribed by RNA polymerase III.
    • 细胞定位Nuclear
    • 数据库链接
    • 别名
      • General transcription factor 3C polypeptide 1 antibody
      • GTF3C1 antibody
      • TF3C-alpha antibody
      • TFIIIC 220 kDa subunit antibody
      • TFIIIC box B-binding subunit antibody
      • TFIIIC220 antibody
      • Transcription factor IIIC-subunit alpha antibody
      see all

    Anti-TFIIIC / GTF3C1 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling TFIIIC / GTF3C1 with ab75251 at 1/1000 (1µg/ml). Detection: DAB.
    • All lanes : Anti-TFIIIC / GTF3C1 antibody (ab75251) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 238 kDa
      Observed band size : 238 kDa


      Exposure time : 3 minutes

    Anti-TFIIIC / GTF3C1 antibody (ab75251)参考文献

    ab75251 has not yet been referenced specifically in any publications.

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