Anti-TFIIIC / GTF3C1抗体(ab37643)

概述

  • 产品名称Anti-TFIIIC / GTF3C1抗体
    参阅全部 TFIIIC / GTF3C1 一抗
  • 描述
    兔多克隆抗体to TFIIIC / GTF3C1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human TFIIIC/ GTF3C1.

    (Peptide available as ab39529.)

  • 阳性对照
    • ab37643 gave a positive result in the following whole cell lysates: Jurkat Jurkat Nuclear HepG2 Nuclear

性能

应用

Our Abpromise guarantee covers the use of ab37643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 238 kDa (predicted molecular weight: 238 kDa).

靶标

Anti-TFIIIC / GTF3C1 antibody 图像

  • All lanes : Anti-TFIIIC / GTF3C1 antibody (ab37643) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 nuclear extract lysate (ab14660)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 238 kDa
    Observed band size : 238 kDa
  • ICC/IF image of ab37643 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37643, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TFIIIC / GTF3C1 antibody (ab37643)参考文献

ab37643 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab37643.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"