产品名称 Anti-TFIIIC / GTF3C1抗体 参阅全部 TFIIIC / GTF3C1 一抗
兔多克隆抗体to TFIIIC / GTF3C1
ICC/IF, WB more details
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human TFIIIC/ GTF3C1.
(Peptide available as
ab37643 gave a positive result in the following whole cell lysates:
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 238 kDa (predicted molecular weight: 238 kDa).
相关性 The transcription factor IIIC (TFIIIC) is a multi-subunit DNA-binding factor required for promoter recognition and TFIIIB assembly on tRNA genes transcribed by RNA polymerase III.
General transcription factor 3C polypeptide 1 antibody
Anti-TFIIIC / GTF3C1 antibody 图像
Western blot - TFIIIC / GTF3C1 antibody (ab37643)
All lanes : Anti-TFIIIC / GTF3C1 antibody (ab37643) at 1 µg/ml Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Jurkat nuclear extract lysate ( ab14844) Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : HepG2 nuclear extract lysate ( ab14660) Lysates/proteins at 10 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 238 kDa Observed band size : 238 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TFIIIC / GTF3C1 antibody (ab37643)
ICC/IF image of ab37643 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37643, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-TFIIIC / GTF3C1 antibody (ab37643)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"