概述

  • 产品名称Anti-TFIIF抗体- ChIP Grade
    参阅全部 TFIIF 一抗
  • 描述
    兔多克隆抗体to TFIIF - ChIP Grade
  • 特异性This antibody reacts with the RAP74 subunit of TFIIF.
  • 经测试应用适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein - RAP 74 subunit of TF11F.

  • 阳性对照
    • HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab28179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Predicted molecular weight: 58 kDa.
ChIP Use 5-10 µg for 25 µg of chromatin.

靶标

  • 相关性The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ATP dependent helicase GTF2F2 antibody
    • BTF 4 antibody
    • BTF4 antibody
    • General transcription factor IIF polypeptide 1 antibody
    • General transcription factor IIF polypeptide 1 74 kDa subunit protein antibody
    • General transcription factor IIF polypeptide 1 74kDa antibody
    • General transcription factor IIF polypeptide 2 30kDa antibody
    • General Transcription Factor IIF Polypeptide 2 antibody
    • GTF2F1 antibody
    • GTF2F1 protein antibody
    • GTF2F2 antibody
    • RAP 30 antibody
    • RAP 74 antibody
    • RAP30 antibody
    • RAP74 antibody
    • TF2F 1 antibody
    • TF2F 2 antibody
    • TF2F1 antibody
    • TF2F2 antibody
    • TFIIF alpha antibody
    • TFIIF beta antibody
    • Transcription initiation factor IIF alpha subunit antibody
    • Transcription Initiation Factor IIF beta Subunit antibody
    • Transcription initiation factor IIF subunit beta antibody
    • Transcription initiation factor RAP30 antibody
    • Transcription initiation factor RAP74 antibody
    see all

Anti-TFIIF antibody - ChIP Grade 图像

  •     Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10mins. The  ChIP was performed with 25µg of chromatin, 8µg of ab28179 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes for the actively transcribed genes GAPDH and ACT1 are located in the core promoter.

Anti-TFIIF antibody - ChIP Grade (ab28179)参考文献

This product has been referenced in:
  • Hogel M  et al. Promoters are differentially sensitive to N-terminal mutant huntingtin-mediated transcriptional repression. PLoS One 7:e41152 (2012). Read more (PubMed: 22815947) »
  • Diamant G  et al. DSIF restricts NF-?B signaling by coordinating elongation with mRNA processing of negative feedback genes. Cell Rep 2:722-31 (2012). WB, ChIP ; Human . Read more (PubMed: 23041311) »

See all 2 Publications for this product

Product Wall

Application ChIP
Sample Human Cell lysate - nuclear (HeLa)
Negative control IgG
Specification HeLa
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control PolII
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Verified customer

提交于 Jul 08 2015

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"