概述

  • 产品名称Anti-TFIIF抗体
    参阅全部 TFIIF 一抗
  • 描述
    兔多克隆抗体to TFIIF
  • 特异性This antibody reacts with Transcription initiation factor IIF beta subunit (GTF2F2), polypeptide 2, 30kDa.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: QLDKVVTTNY KPVANHQYNI EYERKKKEDG KRARADKQHV LDMLFSAFEK, corresponding to internal sequence amino acids 144-193 of Human TFIIF

  • 阳性对照
    • HepG2 cell lysate, Human brain tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab49154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1.5625e+006.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 相关性The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ATP dependent helicase GTF2F2 antibody
    • BTF 4 antibody
    • BTF4 antibody
    • General transcription factor IIF polypeptide 1 antibody
    • General transcription factor IIF polypeptide 1 74 kDa subunit protein antibody
    • General transcription factor IIF polypeptide 1 74kDa antibody
    • General transcription factor IIF polypeptide 2 30kDa antibody
    • General Transcription Factor IIF Polypeptide 2 antibody
    • GTF2F1 antibody
    • GTF2F1 protein antibody
    • GTF2F2 antibody
    • RAP 30 antibody
    • RAP 74 antibody
    • RAP30 antibody
    • RAP74 antibody
    • TF2F 1 antibody
    • TF2F 2 antibody
    • TF2F1 antibody
    • TF2F2 antibody
    • TFIIF alpha antibody
    • TFIIF beta antibody
    • Transcription initiation factor IIF alpha subunit antibody
    • Transcription Initiation Factor IIF beta Subunit antibody
    • Transcription initiation factor IIF subunit beta antibody
    • Transcription initiation factor RAP30 antibody
    • Transcription initiation factor RAP74 antibody
    see all

Anti-TFIIF antibody 图像

  • Anti-TFIIF antibody (ab49154) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling TFIIF with ab49154 at 4-8µg/ml. Arrows indicate positively labelled neural cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spermatophore tissue labelling TFIIF with ab49154 at 4-8µg/ml. Arrows indicate postively labelled epithelial cells. Magnification: 400X.

Anti-TFIIF antibody (ab49154)参考文献

ab49154 has not yet been referenced specifically in any publications.

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