概述

  • 产品名称Anti-TFIIE beta抗体
    参阅全部 TFIIE beta 一抗
  • 描述
    兔多克隆抗体to TFIIE beta
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 242-291 (ISSMQESGPK KVAPIQRRKK PASQKKRRFK THNEHLAGVL KDYSDITSSK) of Human TFIIE beta (NP_002086).

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • 序列相似性Belongs to the TFIIE beta subunit family.
    Contains 1 TFIIE beta DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FE antibody
    • General transcription factor IIE subunit 2 antibody
    • General transcription factor IIE, polypeptide 2, beta 34kDa antibody
    • gtf2e2 antibody
    • T2EB_HUMAN antibody
    • TF2E 2 antibody
    • TF2E2 antibody
    • TFIIE B antibody
    • TFIIE-beta antibody
    • Transcription initiation factor IIE beta subunit antibody
    • Transcription initiation factor IIE subunit beta antibody
    see all

Anti-TFIIE beta antibody 图像

  • Anti-TFIIE beta antibody (ab83484) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

Anti-TFIIE beta antibody (ab83484)参考文献

ab83484 has not yet been referenced specifically in any publications.

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