产品名称 Anti-TFIIE beta抗体 参阅全部 TFIIE beta 一抗
兔多克隆抗体to TFIIE beta
WB, IHC-P, ELISA more details
Synthetic peptide derived from an internal sequence of Human TFIIE beta
Human heart tissue and extracts from Jurkat cells
存放说明 Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
1/50 - 1/100.
功能 Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
序列相似性 Belongs to the TFIIE beta subunit family. Contains 1 TFIIE beta DNA-binding domain.
Information by UniProt
General transcription factor IIE subunit 2 antibody
General transcription factor IIE, polypeptide 2, beta 34kDa antibody
Anti-TFIIE beta antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TFIIE beta antibody (ab64932)
ab64932, at a 1/50 dilution, staining Human TFIIE beta in paraffin-embedded heart tissue, by Immunohistochemistry, with (+) or without (-) immunising peptide.
Western blot - TFIIE beta antibody (ab64932)
All lanes : Anti-TFIIE beta antibody (ab64932) at 1/500 dilution Lane 1 : Extracts from Jurkat cells Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg Lysates/proteins at 5 µg per lane. Predicted band size : 33 kDa Observed band size : 32 kDa ( why is the actual band size different from the predicted?) Additional bands at : 31 kDa,52 kDa. We are unsure as to the identity of these extra bands.
Anti-TFIIE beta antibody (ab64932)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"