Anti-TFIIE alpha抗体- ChIP Grade (ab28177)

概述

  • 产品名称Anti-TFIIE alpha抗体- ChIP Grade
    参阅全部 TFIIE alpha 一抗
  • 描述
    兔多克隆抗体to TFIIE alpha - ChIP Grade
  • 特异性ab28177 recognises TFIIE alpha.
  • 经测试应用适用于: WB, ChIPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human): TFIIE alpha

  • 阳性对照
    • HeLa nuclear extract

性能

应用

Our Abpromise guarantee covers the use of ab28177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
ChIP Use 5-10 µg for 25 µg of chromatin.

靶标

  • 功能Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • 序列相似性Belongs to the TFIIE alpha subunit family.
    Contains 1 HTH TFE/IIEalpha-type domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FE antibody
    • General transcription factor IIE 56 kDa subunit antibody
    • General transcription factor IIE subunit 1 antibody
    • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
    • Gtf2e1 antibody
    • MAL7P1.86 antibody
    • T2EA_HUMAN antibody
    • TF2E1 antibody
    • TFIIE A antibody
    • TFIIE alpha antibody
    • TFIIE-alpha antibody
    • Transcription initiation factor IIE alpha subunit antibody
    • Transcription initiation factor iie alpha subunit putative antibody
    • Transcription initiation factor IIE subunit alpha antibody
    see all

Anti-TFIIE alpha antibody - ChIP Grade 图像

  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).

Anti-TFIIE alpha antibody - ChIP Grade (ab28177)参考文献

ab28177 has not yet been referenced specifically in any publications.

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