Anti-TFIIE alpha抗体(ab99418)

概述

  • 产品名称Anti-TFIIE alpha抗体
    参阅全部 TFIIE alpha 一抗
  • 描述
    兔多克隆抗体to TFIIE alpha
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Other Immunogen Type within Human TFIIE alpha aa 389-439. The exact sequence is proprietary.
    Database link: NP_005504.2

  • 阳性对照
    • HeLa whole cell lysate, 293T whole cell lysate and Mouse NIH3T3 whole cell lysate.
  • 常规说明Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

性能

应用

Our Abpromise guarantee covers the use of ab99418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-TFIIE alpha antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling TFIIE alpha with ab99418 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TFIIE alpha antibody (ab99418) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 49 kDa


    Exposure time : 30 seconds
  • ab99418 at 0.4 µg/ml detecting TFIIE alpha in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 3 second exposure.
    For immunoprecipitation, ab99418 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99416, a rabbit anti-TFIIE alpha antibody which recognizes an upstream epitope
    Lane 2; IP using ab99418.
    Lane 3; IP using control IgG.

Anti-TFIIE alpha antibody (ab99418)参考文献

ab99418 has not yet been referenced specifically in any publications.

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