Anti-TFIIE alpha抗体(ab99416)


  • 产品名称Anti-TFIIE alpha抗体
    参阅全部 TFIIE alpha 一抗
  • 描述
    兔多克隆抗体to TFIIE alpha
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide within Human TFIIE alpha aa 100-150. The exact sequence is proprietary.
    Database link: NP_005504.2

  • 阳性对照
    • HeLa cell lysate
  • 常规说明Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.



Our Abpromise guarantee covers the use of ab99416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
    • 序列相似性Belongs to the TFIIE alpha subunit family.
      Contains 1 HTH TFE/IIEalpha-type domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FE antibody
      • General transcription factor IIE 56 kDa subunit antibody
      • General transcription factor IIE subunit 1 antibody
      • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
      • Gtf2e1 antibody
      • MAL7P1.86 antibody
      • T2EA_HUMAN antibody
      • TF2E1 antibody
      • TFIIE A antibody
      • TFIIE alpha antibody
      • TFIIE-alpha antibody
      • Transcription initiation factor IIE alpha subunit antibody
      • Transcription initiation factor iie alpha subunit putative antibody
      • Transcription initiation factor IIE subunit alpha antibody
      see all

    Anti-TFIIE alpha antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling TFIIE alpha with ab99416 at 1/1000 (1µg/ml). Detection: DAB.
    • ab99418 at 0.4 µg/ml detecting TFIIE alpha in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 3 second exposure.
      For immunoprecipitation, ab99416 and ab99418 were used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
      Lane 1; IP using ab99416
      Lane 2; IP using ab99418 a rabbit anti-TFIIE alpha antibody which recognizes a downstream epitope.
      Lane 3; IP using control IgG.

    Anti-TFIIE alpha antibody (ab99416)参考文献

    ab99416 has not yet been referenced specifically in any publications.

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