Anti-TFIIE alpha抗体(ab43753)

概述

  • 产品名称Anti-TFIIE alpha抗体
    参阅全部 TFIIE alpha 一抗
  • 描述
    小鼠多克隆抗体to TFIIE alpha
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide: EILEPEPTEI PALKQSKDHA ATTAGAASLA GGHHREAWAT KGPSYEDLYT QNVVINMDDQ EDLHRASLEG KSAKERPIWL RESTVQGAYG SEDMKEGGID , corresponding to amino acids 202-302 of Human TFIIE alpha

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protei.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protei.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab43753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 49 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 功能Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • 序列相似性Belongs to the TFIIE alpha subunit family.
    Contains 1 HTH TFE/IIEalpha-type domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FE antibody
    • General transcription factor IIE 56 kDa subunit antibody
    • General transcription factor IIE subunit 1 antibody
    • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
    • Gtf2e1 antibody
    • MAL7P1.86 antibody
    • T2EA_HUMAN antibody
    • TF2E1 antibody
    • TFIIE A antibody
    • TFIIE alpha antibody
    • TFIIE-alpha antibody
    • Transcription initiation factor IIE alpha subunit antibody
    • Transcription initiation factor iie alpha subunit putative antibody
    • Transcription initiation factor IIE subunit alpha antibody
    see all

Anti-TFIIE alpha antibody 图像

  • All lanes : Anti-TFIIE alpha antibody (ab43753) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 500ng of GST fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) conjugated to Horseradish peroxidase at 1/5000 dilution

    Predicted band size : 49 kDa

Anti-TFIIE alpha antibody (ab43753)参考文献

ab43753 has not yet been referenced specifically in any publications.

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