Anti-TFIIE alpha/GTF2E1抗体(ab28177)
Key features and details
- Rabbit polyclonal to TFIIE alpha/GTF2E1
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TFIIE alpha/GTF2E1抗体
参阅全部 TFIIE alpha/GTF2E1 一抗 -
描述
兔多克隆抗体to TFIIE alpha/GTF2E1 -
宿主
Rabbit -
特异性
ab28177 recognises TFIIE alpha/GTF2E1.
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经测试应用
适用于: ChIPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human TFIIE alpha/GTF2E1.
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阳性对照
- HeLa nuclear extract
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.9
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28177于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ChIP | (1) |
Use 5-10 µg for 25 µg of chromatin.
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说明 |
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ChIP
Use 5-10 µg for 25 µg of chromatin. |
靶标
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功能
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. -
序列相似性
Belongs to the TFIIE alpha subunit family.
Contains 1 HTH TFE/IIEalpha-type domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2960 Human
- Omim: 189962 Human
- SwissProt: P29083 Human
- Unigene: 445272 Human
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别名
- FE antibody
- General transcription factor IIE 56 kDa subunit antibody
- General transcription factor IIE subunit 1 antibody
see all
图片
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Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab28177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab28177 被引用在 1 文献中.
- Petkau N et al. Acetylation of BMAL1 by TIP60 controls BRD4-P-TEFb recruitment to circadian promoters. Elife 8:N/A (2019). PubMed: 31294688